仙山湖国家湿地公园土壤中固碳微生物的群落与功能基因特征

陆章欣; 严飞扬; 吴酬飞; 史嘉仪; 李鹏; 赵明星; 张宪翠; 曾慧慧; 张峰

doi:10.13961/j.cnki.stbctb.2025.01.025

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仙山湖国家湿地公园土壤中固碳微生物的群落与功能基因特征

试验研究

- 仙山湖国家湿地公园土壤中固碳微生物的群落与功能基因特征
- Community and functional gene characteristics of soil carbon sequestration microorganisms in Xianshanhu National Wetland Park
- 水土保持通报 2025年45卷第1期页码：235-243
- 作者机构：
  
  1. 湖州师范学院,浙江,湖州,313000
  2. 浙江省森林资源监测中心,浙江,杭州,310020
  3. 杭州鸿森林业调查规划设计有限公司,浙江,杭州,310020
- 作者简介：
- 基金信息：
  
  长兴县自然资源和规划局科学研究项目“仙山湖国家湿地公园固碳增汇能力提升关键技术研发与示范”(ZJXRCX-2022-012);浙江省大学生科技创新活动计划暨新苗人才计划(2023R462022)
- DOI：10.13961/j.cnki.stbctb.2025.01.025
  中图分类号： Q176;Q178;X171.1
- 纸质出版：2025
- 稿件说明：
移动端阅览
陆章欣, 严飞扬, 吴酬飞, 等. 仙山湖国家湿地公园土壤中固碳微生物的群落与功能基因特征[J]. 水土保持通报, 2025,45(1):235-243.

Lu Zhangxin, Yan Feiyang, Wu Choufei, et al. Community and functional gene characteristics of soil carbon sequestration microorganisms in Xianshanhu National Wetland Park[J]. Bulletin of Soiland Water Conservation, 2025, 45(1): 235-243.
陆章欣, 严飞扬, 吴酬飞, 等. 仙山湖国家湿地公园土壤中固碳微生物的群落与功能基因特征[J]. 水土保持通报, 2025,45(1):235-243. DOI： 10.13961/j.cnki.stbctb.2025.01.025.

Lu Zhangxin, Yan Feiyang, Wu Choufei, et al. Community and functional gene characteristics of soil carbon sequestration microorganisms in Xianshanhu National Wetland Park[J]. Bulletin of Soiland Water Conservation, 2025, 45(1): 235-243. DOI： 10.13961/j.cnki.stbctb.2025.01.025.

摘要

[目的

]

分析湿地不同土地利用方式下0—20 cm土壤性质和微生物菌群结构变化，探究不同土地利用方式下土壤固碳微生物群落与功能基因变化特征，为湿地生态系统资源的保护和可持续利用提供科学参考。[方法

]

以浙江长兴仙山湖国家湿地公园为研究对象。运用高通量测序技术对基因进行测序分析。[结果

]

①仙山湖湿地固碳能力表现为：莎草沼泽＞天然沿岸森林＞竹林＞苗圃＞人为干预的河道沿岸森林(落羽杉树林)＞人工松树林＞阔叶林＞人为干预的沿岸植被＞人为干预的河道沿岸森林(柳树沼泽)＞芦苇沼泽＞芦苇和柳树沿岸植被，莎草沼泽有机碳储量最高(38.68±0.56 t/hm

)，为森林有机碳储量的171%，其土壤微生物碳利用效率(0.66±0.005)为竹林微生物碳利用效率的150%。②仙山湖湿地微生物固碳途径主要以卡尔文循环，还原性三羧酸循环和还原乙酰辅酶A途径为主，变形菌门、拟杆菌门、酸杆菌门、绿弯菌门为主要固碳菌群。莎草沼泽土壤变形菌门相对丰度为56.40%，拟杆菌门相对丰度为17.40%，天然沿岸森林土壤变形菌门相对丰度为59.60%，竹林土壤酸杆菌门相对丰度为36.00%，仙山湖湿地有机碳储量与土壤固碳菌门丰度成正相关关系。[结论

]

仙山湖湿地生态系统中固碳微生物的优势菌群差异大，不同土地利用方式下土壤固碳微生物群落与功能基因变化影响着湿地微生物固碳能力。其中，莎草沼泽中土壤有机碳储量、全氮含量和微生物碳利用效率最高，其固碳功能基因高于其他土地利用方式。因此，莎草沼泽具有较高的碳储存能力。

Abstract

[Objective] Changes in soil properties and microbial community structure in 0—20 cm soil under different land use modes in wetlands were analyzed

and the characteristics of soil carbon sequestration microbial communities and functional gene changes under different land use modes were explored to provide scientific references for the protection and sustainable utilization of wetland ecosystem resources. [Methods] The Xianshan Lake National Wetland Park in Changxing

Zhejiang Province was taken as the research object. High-throughput sequencing technology was used to sequence the genes. [Results] ① The carbon sequestration capacity of the Xianshan Lake wetland was as follows: sedge swamp>natural coastal forest>bamboo forest>nursery>artificially intervened riverbank forest (Chinese fir forest)>artificial pine forest>broad-leaved forest>artificially intervened coastal vegetation>artificially intervened riverbank forest (willow swamp)>reed swamp>reed and willow coastal vegetation. The organic carbon storage of sedge swamp was the highest (38.68±0.56 t/hm2)

accounting for 171% of forest organic carbon storage

and its soil microbial carbon utilization efficiency (0.66±0.005) was 150% of bamboo forest microbial carbon utilization efficiency. ② The carbon sequestration pathways of microorganisms in the Xianshan Lake wetland were mainly facilitated through the Calvin cycle

reducing the tricarboxylic acid cycle and reducing acetyl CoA pathway

with Proteobacteria

Bacteroidetes

Acidobacteria

and Chloroflexia as the main carbon sequestration microbial communities. The relative abundance of Proteobacteria and Bacteroidetes in the sedge swamp soil were 56.40% and 17.40%

respectively. The relative abundance of Proteobacteria in the natural coastal forest soil was 59.60%

and that of Acidobacteria in the bamboo forest soil was 36.00%. There was a positive correlation between organic carbon storage and soil carbon-fixing bacterial abundance in the Xianshan Lake wetland. [Conclusion] The dominant microbial communities for carbon sequestration in the Xianshan Lake wetland ecosystem varied greatly

and changes in soil carbon sequestration microbial communities and functional genes under different land use patterns affected the carbon sequestration capacity of wetland microorganisms. Among them

soil organic carbon storage

total nitrogen content

and microbial carbon utilization efficiency were the highest in the sedge swamp

and its carbon sequestration functional genes were higher than those of other land-use methods. Therefore

sedge marshes have a high carbon storage capacity.

关键词

Keywords

references

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