沙柳沙障腐烂过程真菌多样性及其对环境因子的响应

梁钰镁; 高永; 蒙仲举; 阿拉腾陶格苏

doi:10.13961/j.cnki.stbctb.2023.05.003

您当前的位置：

首页 >

文章列表页 >

沙柳沙障腐烂过程真菌多样性及其对环境因子的响应

试验研究

- 沙柳沙障腐烂过程真菌多样性及其对环境因子的响应
- Fungal Diversity and Its Response to Environmental Factors During Decay Process of Salix Psammophila Sand Barriers
- 水土保持通报 2023年43卷第5期页码：18-26
- 作者机构：
  
  1. 内蒙古农业大学沙漠治理学院,内蒙古,呼和浩特,010018
  2. 鄂尔多斯市地质调查监测院,内蒙古,鄂尔多斯,017000
- 作者简介：
- 基金信息：
  
  国家重点研发计划专题“沙区林光互补—防风固沙—沙产业一体化生态修复及生态效益评估技术”(2022YFF1303404)
- DOI：10.13961/j.cnki.stbctb.2023.05.003
  中图分类号： S775
- 纸质出版：2023
- 稿件说明：
移动端阅览
梁钰镁, 高永, 蒙仲举, 等. 沙柳沙障腐烂过程真菌多样性及其对环境因子的响应[J]. 水土保持通报, 2023,43(5):18-26.

Liang Yumei, Gao Yong, Meng Zhongju, et al. Fungal Diversity and Its Response to Environmental Factors During Decay Process of Salix Psammophila Sand Barriers[J]. Bulletin of Soiland Water Conservation, 2023, 43(5): 18-26.
梁钰镁, 高永, 蒙仲举, 等. 沙柳沙障腐烂过程真菌多样性及其对环境因子的响应[J]. 水土保持通报, 2023,43(5):18-26. DOI： 10.13961/j.cnki.stbctb.2023.05.003.

Liang Yumei, Gao Yong, Meng Zhongju, et al. Fungal Diversity and Its Response to Environmental Factors During Decay Process of Salix Psammophila Sand Barriers[J]. Bulletin of Soiland Water Conservation, 2023, 43(5): 18-26. DOI： 10.13961/j.cnki.stbctb.2023.05.003.

摘要

[目的] 分析沙柳沙障腐烂过程对真菌群落组成及多样性的影响，为进一步探究荒漠生态系统真菌群落的生态功能提供理论依据。[方法] 选取1，3，5，7a的沙柳沙障为研究对象，新铺设的沙障作为对照，采用野外原位取样、室内指标测定和Illumina MiSeq测序技术，揭示沙柳沙障腐烂过程中真菌多样性与沙障成分、土壤性质及酶活性之间的响应关系。[结果] ①瓶霉属、木霉属、Knufia属、维罗纳霉属和锥毛壳属均呈上升趋势，但亚隔孢壳属和Phaeococcomyces属呈下降趋势，真菌多样性与丰富度整体表现为增加。②7a的有机碳、碱解氮和可溶性有机碳分别是对照的2.47，1.83，1.71倍，β-1，4-葡萄糖苷酶和亮氨酸氨基肽酶活性分别增加了139.36%和35.16%。③Knufia属、近地伞属、维罗纳霉属和锥毛壳属分别与有机碳，碱解氮和可溶性有机碳呈显著正相关，但与纤维素和半纤维呈显著负相关(p＜0.05)。冗余RDA分析结果表明，纤维素和碱解氮是影响优势菌群丰度的主要因子，碱解氮和亮氨酸氨基肽酶是影响多样性指数的主要因子。[结论] 在沙柳沙障腐烂过程中，优势真菌属和多样性均受到多种因子的综合影响，碱解氮为两者的主要影响因子。在未来的研究中需进一步结合FUNGuild数据库探讨优势真菌群所具有的生态功能。

Abstract

[Objective] The effects of Salix psammophila sand barrier decay on the composition and diversity of fungal communities were analyzed in order to provide a theoretical basis for further research on the ecological function of fungal communities in desert ecosystems. [Methods] S. psammophila sand barriers aged 1

and 7 years were observed

and newly laid sand barriers were used as the control. Field in-situ sampling

laboratory index determination

and Illumina MiSeq sequencing technology were used to determine the response relationships between fungal diversity and sand barrier components

soil properties

and enzyme activities during sand barrier decay. [Results] ① The relative abundance of Phialophora

Trichoderma

Knufia

Veronaea and Coniochaeta increased over time

while Didymella and Phaeococcomyces decreased over time. Fungal diversity and richness increased over time. ② Soil organic carbon

alkaline nitrogen

and dissolved organic carbon at seven years were 2.47

1.83

and 1.71 times higher

respectively

than observed for the control. Activities of β-1

4-glucosidase and leucine aminopeptidase significantly increased by 139.36% and 35.16%

respectively. ③ Knufia

Parasola

Veronaea

and Coniochaeta were positively correlated with soil organic carbon

alkaline nitrogen

and dissolved orgainic carbon

but negatively correlated with cellulose and hemifiber (p＜0.05). The results of the redundancy analysis showed that cellulose and alkaline nitrogen were the main environmental factors that significantly affected the abundance of dominant fungal communities

and alkaline nitrogen and leucine aminopeptidase were the main factors that influenced the diversity index. [Conclusion] Both the dominant fungal genera and diversity were affected by a combination of factors during S. psammophila sand barrier decay. Alkaline nitrogen was the main influencing factor for both dominant fungal genera and diversity. Future studies should further explore the ecological functions of the dominant fungal groups in combination with the FUNGuild database.

关键词

Keywords

references

高永,虞毅,龚萍.沙柳沙障[M].北京:科学出版社,2013.

曹波,孙保平,高永,等.高立式沙柳沙障防风效益研究[J].中国水土保持科学,2007,5(2):40-45.

蒙仲举,任晓萌,高永.半隐蔽式沙柳沙障的防风阻沙效益[J].水土保持通报,2014,34(3):178-180.

Wang Ruidong, Gao Yong, Dang Xiaohong, et al. Micro-structure and biodegradation of long-established Salix psammophila sand barriers on sand dunes [J]. Environmental Technology & Innovation, 2021,21:101366.

龚萍.沙柳沙障腐蚀过程及防腐抗蚀效果研究[D].内蒙古呼和浩特:内蒙古农业大学,2012.

Boddy L. Fungal community ecology and wood decomposition processes in angiosperms: from standing tree to complete decay of coarse woody debris[J]. Ecological Bulletins, 2001,49:43-56.

Hoppe B, Purahong W, Wubet T, et al. Linking molecular deadwood-inhabiting fungal diversity and community dynamics to ecosystem functions and processes in Central European forests [J]. Fungal Diversity,2016,77(1):367-379.

Buchan A, Newell S Y, Butler M, et al. Dynamics of bacterial and fungal communities on decaying salt marsh grass [J]. Applied and Environmental Microbiology,2003,69(11):6676-6687.

Torzilli A P, Sikaroodi M, Chalkley D, et al. A comparison of fungal communities from four salt marsh plants using automated ribosomal intergenic spacer analysis (ARISA) [J]. Mycologia, 2006,98(5):690-698.

Nicholas D D, Crawford D. Concepts in the development of new accelerated test methods for wood decay[M]//Goodell B, Nicholas D D, Schultz T P (eds). ACS Symposium Series. Washington, DC: American Chemical Society, 2003:288-312.

Rayner A D M, Boddy L. Fungal decomposition of wood: its biology and ecology [M]. Chichester: Wiley, 1988.

van der Wal A, de Boer W, Smant W, et al. Initial decay of woody fragments in soil is influenced by size, vertical position, nitrogen availability and soil origin [J]. Plant and Soil, 2007,301(1):189-201.

Wang Ruidong, Yang Xia, Gao Yong, et al. Decomposition characteristics of long-established

Salix psammophila

sand barriers in an arid area, Northwestern China [J]. BioResources, 2021,16(3):5947-5963.

万红云,陈林,庞丹波,等.贺兰山不同海拔土壤酶活性及其化学计量特征[J].应用生态学报,2021,32(9):3045-3052.

梁钰镁,高永,党晓宏,等.不同年限沙柳沙障沙埋部腐化特性及真菌群落特征[J].应用与环境生物学报,2022,28(2):339-345.

孙悦.NADH脱氢酶调控丝状真菌

Podospora anserina

降解木质纤维素及子实体发育研究[D].广东深圳:深圳大学,2020.

Purahong W, Wubet T, Lentendu G, et al. Life in leaf litter: novel insights into community dynamics of bacteria and fungi during litter decomposition [J]. Molecular Ecology, 2016,25(16):4059-4074.

赵军旗.瓶霉属

Phialophora

spp.来源的纤维素酶和半纤维素酶的基因克隆与表达[D].北京:中国农业科学院,2012.

Rytioja J, Hildén K, Yuzon J, et al. Plant-polysaccharide-degrading enzymes from basidiomycetes [J]. Microbiology and Molecular Biology Reviews, 2014,78(4):614-649.

Mehrabi M, Asgari B, Hemmati R.

Knufia perfecta

, a new black yeast from Iran, and a key to

Knufia

species [J]. Nova Hedwigia, 2018,106(3/4):519-534.

Rajala T, Peltoniemi M, Pennanen T, et al. Fungal community dynamics in relation to substrate quality of decaying Norway spruce (

Picea abies

Karst.) logs in boreal forests [J]. FEMS Microbiology Ecology, 2012,81(2):494-505.

Bani A, Pioli S, Ventura M, et al. The role of microbial community in the decomposition of leaf litter and deadwood [J]. Applied Soil Ecology,2018,126:75-84.

Gianfreda L, Ruggiero P. Enzyme activities in soil [M]//Nannipieri P, Smalla K (eds). Soil Biology. Heidelberg: Springer Berlin Heidelberg, 2006:257-311.

Sinsabaugh R L, Hill B H, Follstad Shah J J. Ecoenzymatic stoichiometry of microbial organic nutrient acquisition in soil and sediment [J]. Nature, 2009,462(7274):795-798.

Sawada K, Inagaki Y, Sugihara S, et al. Impacts of conversion from natural forest to cedar plantation on the structure and diversity of root-associated and soil microbial communities [J]. Applied Soil Ecology, 2021,167:104027.

Fioretto A, Di Nardo C, Papa S, et al. Lignin and cellulose degradation and nitrogen dynamics during decomposition of three leaf litter species in a Mediterranean ecosystem [J]. Soil Biology and Biochemistry, 2005,37(6):1083-1091.

Frey S D, Elliott E T, Paustian K, et al. Fungal translocation as a mechanism for soil nitrogen inputs to surface residue decomposition in a no-tillage agroecosystem [J]. Soil Biology and Biochemistry, 2000,32(5):689-698.

浏览量

868

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

高寒矿区排土场边坡植被恢复年限对产流产沙的影响

旅游干扰对贺兰山典型草原生物多样性及土壤性质的影响

紫色土坡地土壤性质对耕作侵蚀的影响

黄土区梯化坡地不同土地利用方式对土壤理化性质的影响

库布齐沙漠北缘机械沙障对地表土壤可蚀性的影响