黑麦草生长及根系形态对土壤Cd，Pb与石油污染的响应

魏倩; 周际海; 程双怀; 黄永杰; 张杰; 周守标

doi:10.13961/j.cnki.stbctb.2021.02.011

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黑麦草生长及根系形态对土壤Cd，Pb与石油污染的响应

试验研究

- 黑麦草生长及根系形态对土壤Cd，Pb与石油污染的响应
- Response of Ryegrass Growth and Root Morphology to Soil Cd, Pb and Petroleum Pollution
- 水土保持通报 2021年41卷第2期页码：84-91
- 作者机构：
  
  1. 安徽师范大学生命科学学院安徽省重要生物资源保护与利用研究重点实验室,安徽,芜湖,241000
  2. 南昌工程学院江西省退化生态系统修复与流域生态水文重点实验室,江西,南昌,330099
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目“食用菌线虫调控细菌群落影响污染土壤石油降解的机理研究”（31460149），“秸秆及其生物质炭还田提升旱地红壤地力的差异与机理研究”（41661065）;江西省教育厅科学技术研究项目重点项目（GJJ180921）
- DOI：10.13961/j.cnki.stbctb.2021.02.011
  中图分类号： S543.6;X173
- 纸质出版：2021
- 稿件说明：
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魏倩, 周际海, 程双怀, 等. 黑麦草生长及根系形态对土壤Cd，Pb与石油污染的响应[J]. 水土保持通报, 2021,41(2):84-91.

Wei Qian, Zhou Jihai, Cheng Shuanghuai, et al. Response of Ryegrass Growth and Root Morphology to Soil Cd, Pb and Petroleum Pollution[J]. Bulletin of Soiland Water Conservation, 2021, 41(2): 84-91.
魏倩, 周际海, 程双怀, 等. 黑麦草生长及根系形态对土壤Cd，Pb与石油污染的响应[J]. 水土保持通报, 2021,41(2):84-91. DOI： 10.13961/j.cnki.stbctb.2021.02.011.

Wei Qian, Zhou Jihai, Cheng Shuanghuai, et al. Response of Ryegrass Growth and Root Morphology to Soil Cd, Pb and Petroleum Pollution[J]. Bulletin of Soiland Water Conservation, 2021, 41(2): 84-91. DOI： 10.13961/j.cnki.stbctb.2021.02.011.

摘要

[目的

]

研究不同污染条件下黑麦草生理指标的动态变化，为植物修复污染土壤提供初步理论基础。[方法

]

通过室内试验模拟不同类型的土壤污染。设置4个处理，未污染土壤+黑麦草（SH）、重金属污染土壤（500 mg/kg Pb

和50 mg/kg Cd

）+黑麦草（SGH）、石油污染土壤（1 000 mg/kg石油）+黑麦草（SPH）、石油和重金属复合污染土壤（500 mg/kg Pb

，50 mg/kg Cd

和1 000 mg/kg石油）+黑麦草（SPGH），采用WINRHIZO根系分析系统测量根系形态指标，用分光光度计测量叶片色素指标，研究不同类型土壤污染对黑麦草生长的影响。[结果

]

3种不同类型的污染土壤均不同程度地刺激了黑麦草根系的生长。与未污染处理相比，在20 d时，石油重金属复合处理下的黑麦草根系的根长、根表面积、根体积、根直径分别增加了88.10%，148.60%，221.90%，32.20%。与未污染相比，随着培养时间的增加各污染处理的地下生物量呈现出先增加后减少的趋势，地上生物量均低于未污染处理，且在第10，40，80 d时，复合污染处理的地上生物量最小，较未污染处理分别降低了34.68%，45.42%，58.05%。80 d时，重金属污染处理、石油污染处理和石油重金属复合污染处理的黑麦草叶绿素含量显著低于未污染处理，分别降低了26.84%，44.82%和47.02%。[结论

]

不同污染物都可以促进黑麦草根系形态发育，降低黑麦草的生物量及色素含量；石油重金属复合处理对黑麦草的生长影响最大，石油污染处理次之。基于不同污染物对黑麦草根系形态及生长影响的差异，在今后植物修复土壤污染过程中，可添加一些生长调节物质来缓解污染对植物的毒害作用，提高植物生物量及增强相关生理功能，提升污染土壤修复效果。

Abstract

[Objective] The dynamic changes of physiological indexes of ryegrass under different pollution conditions were studied

in order to provide a preliminary theoretical basis for phytoremediation of contaminated soil.[Methods] Through indoor simulation of different types of soil pollution

4 treatments were set up

namely uncontaminated soil + ryegrass (SH) as contorl

heavy metal contaminated soil (500 mg/kg Pb2+

50 mg/kg Cd2+)+ ryegrass (SGH)

petroleum contaminated soil (1 000 mg/kg petroleum) + ryegrass (SPH)

petroleum and heavy metals combined contaminated soil (500 mg/kg Pb2+

50 mg/kg Cd2+

1 000 mg/kg petroleum) + ryegrass (SPGH). The root morphological index was measured by WINRHIZO root analysis system

and the pigment index of leaves was measured by spectrophotometer

to explore the influence of different types of soil pollution on the pigment content of ryegrass leaves.[Results] The root growth of ryegrass was stimulated by three different types of contaminated soil to varying degrees. Compared with control

on the 20 th day

the root length

root surface area

root volume

and root diameter of plant roots under the combined treatment of petroleum and heavy metals increased by 88.10%

148.60%

221.90%

and 32.20%

respectively. Compared with control

the underground biomass of each polluted treatment showed a trend of increasing firstly and then decreasing with the increase of cultivation time

while the above-ground biomass was always lower than that of control. Moreover

on the 10th

40th and 80th days

the abovementioned biomass of the combined pollutted treatment was the smallest

which significantly decreased by 34.68%

45.42% and 58.05% compared with that of the control. On the 80 th day

the chlorophyll content of ryegrass in heavy metal contaminated soil

petroleum contaminated soil and petroleum and heavy metals combined contaminated soil was significantly lower than that of control

which decreased by 26.84%

44.82% and 47.02%

respectively.[Conclusion] Different pollutions could promote the morphological changes of ryegrass root system

and reduce the biomass and pigment contents of ryegrass

among which the combined pollution with petroleum and heavy metals have the greatest influence on the growth of ryegrass

followed by the treatment with petroleum pollution. Based on the difference of physiological response of ryegrass root morphology and growth to different polluted soils

some regulatory substances can be added to alleviate the toxic effect of pollution on plants in the future during the phytoremediation of contaminated soils

increase the biomass of plants and enhance the related physiological functions to improve the efficiency of pollution remediation.

关键词

Keywords

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