油污胁迫下土壤微生物群落结构对外源施用玉米秸秆生物质炭的响应

王金成; 井明博; 张绍鹏; 周天林; 刘光琇; 陈拓; 吴胜伟

doi:10.13961/j.cnki.stbctb.2020.03.010

您当前的位置：

首页 >

文章列表页 >

油污胁迫下土壤微生物群落结构对外源施用玉米秸秆生物质炭的响应

试验研究

- 油污胁迫下土壤微生物群落结构对外源施用玉米秸秆生物质炭的响应
- Response of Soil Microbial Community Structure to Application of Corn Straw Biochar Under Crude-Oil Pollution Concentration Stress
- 水土保持通报 2020年40卷第3期页码：64-74
- 作者机构：
  
  1. 陇东学院生命科学与技术学院,甘肃,庆阳,745000
  2. 甘肃省高校陇东生物资源保护与利用省级重点实验室,甘肃,庆阳,745000
  3. 甘肃省极端环境微生物资源与工程重点实验室中国科学院西北生态环境资源研究院,甘肃,兰州,730000
  4. 中国石油天然气股份有限公司长庆油田分公司第十一采油厂,甘肃,庆阳,745000
  5. 西安环发生物科技有限公司,陕西,西安,710068
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目“陇东黄土高原植物微生物组合联合修复石油污染土壤降解机制研究”（31860148）;科技部国家国际合作专项“青藏高原冻土中原油降解微生物的筛选及耐冷工程菌构建”（2014DFA30330）;陇东学院博士基金项目（XYBY1909）;甘肃省青年科技基金计划项目（17JR5RM354）;甘肃省极端环境微生物资源与工程重点实验室开放基金（EEMRE201601）。
- DOI：10.13961/j.cnki.stbctb.2020.03.010
  中图分类号： X53;S154.3
- 纸质出版：2020
- 稿件说明：
移动端阅览
王金成, 井明博, 张绍鹏, 等. 油污胁迫下土壤微生物群落结构对外源施用玉米秸秆生物质炭的响应[J]. 水土保持通报, 2020,40(3):64-74.

Wang Jincheng, Jing Mingbo, Zhang Shaopeng, et al. Response of Soil Microbial Community Structure to Application of Corn Straw Biochar Under Crude-Oil Pollution Concentration Stress[J]. Bulletin of Soiland Water Conservation, 2020, 40(3): 64-74.
王金成, 井明博, 张绍鹏, 等. 油污胁迫下土壤微生物群落结构对外源施用玉米秸秆生物质炭的响应[J]. 水土保持通报, 2020,40(3):64-74. DOI： 10.13961/j.cnki.stbctb.2020.03.010.

Wang Jincheng, Jing Mingbo, Zhang Shaopeng, et al. Response of Soil Microbial Community Structure to Application of Corn Straw Biochar Under Crude-Oil Pollution Concentration Stress[J]. Bulletin of Soiland Water Conservation, 2020, 40(3): 64-74. DOI： 10.13961/j.cnki.stbctb.2020.03.010.

摘要

[目的

]

分析外源施用玉米秸秆生物质炭对石油污染土壤酶活性和微生物群落结构的影响方式，旨在为黄土高原陇东地区油污土壤生态修复提供新的土壤调理剂。[方法

]

对轻度（5%）和重度（20%）油污土壤分别实施了玉米秸秆生物炭（B）、金盏菊（J）和金盏菊+玉米秸秆生物炭（JB）3种处理方式，采用常规方法测定了不同处理组间土壤总石油烃（TPH）去除率、酶活性和土壤微生物群落结构。[结果

]

①轻度污染时土壤总石油烃（TPH）去除率为JB

（61.95±1.39%）最高，重度污染时为JB

（56.44±1.89%）最高（p

0.05）；②外源施用玉米秸秆生物质炭能有效增加轻重两种油污浓度的土壤脱氢酶和多酚氧化酶活性；③油污浓度由5%增至20%，两种生物质炭参与的处理组（JB

和B

）土壤微生物群落结构组成差异相对较小（p

0.05）；④非度量多维尺度（NMDS）分析结果显示，土壤脱氢酶、多酚氧化酶、Chao1指数和Shannon指数是驱动J

，B

，JB

和JB

处理组土壤微生物群落结构分异于CK

，CK

和J

处理组的主要环境因子。[结论

]

轻重两种油污浓度胁迫时外源施用玉米秸秆生物质炭可显著增加土壤脱氢酶和多酚氧化酶活性，驱动土壤微生物群落结构变化，从而提高了土壤TPH去除率，因此陇东黄土高原地区油污土壤场地生态修复时可选用玉米秸秆生物质炭作为土壤调理剂。

Abstract

[Objective] The effects of exogenous application of corn straw biochar on the soil enzyme activity and microbial community structure were studied to provide soil conditioner for ecological restoration of crude-oil-contaminated soil in Longdong area of the Loess Plateau.[Methods] Three treatments

namely corn straw biochar treatment (B)

Calendula officinalis treatment (J)

and Calendula officinalis plus corn straw biochar treatment (JB) were applied to the low-concentration (5%) and high-concentration (20%) crude-oil-polluted soil. The change of the soil total petroleum hydrocarbon (TPH) removal rate

the soil enzyme activity

and the soil microbial community structure was investigated by the conventional method.[Results] ① The maximum soil TPH removal rates for the low-pollution-concentration group (TPH=5%) were observed in JB5 treatment (61.95%±1.39%)

whereas the JB20 treatment (56.44%±1.89%) demonstrated the maximum soil TPH removal rate

in the high-pollution-concentration (TPH=20%) group (p<0.05). ② Soil dehydrogenase and polyphenol oxidase activity significantly improved after the application of corn straw biochar. ③ When the crude-oil concentration increased from 5% to 20%

the difference in soil microbial community structure between JB20 and B20 treatments was relatively small. ④ Nonmetric multidimensional scaling analysis revealed that the difference in soil microbial community structure between the treatments of J5

B20

JB5

and JB20 and the treatments of CK5

CK20

and J20 was caused by the changes of soil dehydrogenase activity

soil polyphenol oxidase activity

Chao1 index

and Shannon-Wiener index of the soil microbial community.[Conclusion] Application of corn straw biochar to crude-oil-polluted soil with low and high concentrations significantly improved the soil dehydrogenase and polyphenol oxidase activity

causing changes in the soil microbial community structure. Consequently

the soil TPH removal rate improved. Therefore

corn straw biochar can be used as a soil conditioner for ecological restoration of crude-oil-contaminated soil in Longdong area of the Loess Plateau.

关键词

Keywords

references

Barati M, Bakhtiari F, Mowla D, et al. Total petroleum hydrocarbon degradation in contaminated soil as affected by plants growth and biochar [J]. Environmental Earth Sciences, 2017,76(20):688-699.

王金成,井明博,段春艳,等.陇东黄土高原石油污染土壤环境因子对金盏菊(

Calendula officinalis

)-微生物联合修复的响应[J].环境科学学报,2015,35(9):2971-2981.

王金成,井明博,段春燕,等.石油烃污染对陇东黄土高原土壤生物学及非生物学特性的影响[J].水土保持通报,2017,37(1):9-16.

Cao Yanan, Yang Baoshan, Song Ziheng, et al. Wheat straw biochar amendments on the removal of polycyclic aromatic hydrocarbons (PAHs) in contaminated soil [J]. Ecotoxicology and Environmental Safety, 2016, 130: 248-255.

Zeng Guangming, Wu Haipeng, Liang Jie, et al. Efficiency of biochar and compost (or composting) combined amendments for reducing Cd, Cu, Zn and Pb bioavailability, mobility and ecological risk in wetland soil [J]. RSC Advances, 2015,5(44):34541-34548.

González-Chávez M C A, Carrillo-González R, Hernández Godínez M I, et al. Jatropha curcas and assisted phytoremediation of a mine tailing with biochar and a mycorrhizal fungus [J]. International Journal of Phytoremediation, 2017,19(2):174-182.

Zhang Manyun, Wang Jun, Bai S H, et al. Assisted phytoremediation of a co-contaminated soil with biochar amendment: Contaminant removals and bacterial community properties [J]. Geoderma, 2019,348:115-123.

Ni N, Wang Fang, Song Yang, et al. Mechanisms of biochar reducing the bioaccumulation of PAHs in rice from soil: Degradation stimulation vs immobilization [J]. Chemosphere, 2018,196:288-296.

Herrmann L, Lesueur D, Robin A, et al. Impact of biochar application dose on soil microbial communities associated with rubber trees in North East Thailand [J]. Science of the Total Environment, 2019,689:970-979.

朱文英，唐景春.小麦秸秆生物炭对石油烃污染土壤的修复作用[J].农业资源与环境学报,2014,31(3):259-264.

Bao Yunjuan, Xu Zixiang, Li Yang, et al. High-throughput metagenomic analysis of petroleum-contaminated soil microbiome reveals the versatility in xenobiotic aromatics metabolism [J]. Journal of Environmental Sciences, 2017,56:25-35.

Jiao Shuo, Liu Zhenshan, Lin Yanbing, et al. Bacterial communities in oil contaminated soils: Biogeography and co-occurrence patterns [J]. Soil Biology and Biochemistry, 2016,98:64-73.

Saum L, Jiménez M B, Crowley D. Influence of biochar and compost on phytoremediation of oil-contaminated soil [J]. International Journal of Phytoremediation, 2018,20(1):54-60.

Han Tao, Zhao Zhipeng, Bartlam M, et al. Combination of biochar amendment and phytoremediation for hydrocarbon removal in petroleum-contaminated soil [J]. Environmental Science and Pollution Research, 2016,23(21):21219-21228.

Wang Yanjie, Li Fayun, Rong Xiangmin, et al. Remediation of petroleum-contaminated soil using bulrush straw powder, biochar and nutrients [J]. Bulletin of Environmental Contamination and Toxicology, 2017,98(5):690-697.

Zhen Meinan, Chen Hongkun, Liu Qinglong, et al. Combination of rhamnolipid and biochar in assisting phytoremediation of petroleum hydrocarbon contaminated soil using Spartina anglica [J]. Journal of Environmental Sciences, 2019,85:107-118.

Al-Baldawi I A, Abdullah S R S, Anuar N, et al. Phytodegradation of total petroleum hydrocarbon (TPH) in diesel-contaminated water using Scirpus grossus [J]. Ecological Engineering, 2015,74:463-473.

Moubasher H A, Hegazy A K, Mohamed N H, et al. Phytoremediation of soils polluted with crude petroleum oil using Bassia scoparia and its associated rhizosphere microorganisms [J]. International Biodeterioration & Biodegradation, 2015,98:113-120.

Chintala R, Mollinedo J, Schumacher T E, et al. Effect of biochar on chemical properties of acidic soil [J]. Archives of Agronomy and Soil Science, 2014,60(3):393-404.

Ameloot N, Graber E R, Verheijen F G A, et al. Interactions between biochar stability and soil organisms: Review and research needs [J]. European Journal of Soil Science, 2013,64(4):379-390.

王忠强, 刘婷婷, 孟宪民, 等. 泥炭保护紫花苜蓿根系对柴油污染土壤修复的研究[J]. 环境科学学报, 2007,27(3):421-425.

Oliveira F R, Patel A K, Jaisi D P, et al. Environmental application of biochar: Current status and perspectives [J]. Bioresource Technology, 2017,246:110-122.

Chen Ming, Xu Piao, Zeng Guangming, et al. Bioremediation of soils contaminated with polycyclic aromatic hydrocarbons, petroleum, pesticides, chlorophenols and heavy metals by composting: Applications, microbes and future research needs [J]. Biotechnology Advances, 2015,33(6):745-755.

Lee D W, Lee H, Lee A H, et al. Microbial community composition and PAHs removal potential of indigenous bacteria in oil contaminated sediment of Taean Coast, Korea [J]. Environmental Pollution, 2018,234:503-512.

Sutton N B, Maphosa F, Morillo J A, et al. Impact of long-term diesel contamination on soil microbial community structure [J]. Applied and Environmental Microbiology, 2013,79(2):619-630.

Li Xiaojing, Li Yue, Zhang Xiaolin, et al. Long-term effect of biochar amendment on the biodegradation of petroleum hydrocarbons in soil microbial fuel cells [J]. Science of the Total Environment, 2019,651:796-806.

Visioli G, Sanangelantoni A M, Vamerali T, et al. 16S rDNA profiling to reveal the influence of seed-applied biostimulants on the rhizosphere of young maize plants [J]. Molecules, 2018,23(6):1461.

Obafemi Y D, Taiwo O S, Omodara O J, et al. Biodegradation of crude petroleum by bacterial consortia from oil-contaminated soils in Ota, Ogun State, South-Western, Nigeria [J]. Environmental Technology & Innovation, 2018,12:230-242.

王宝山,温成成,孙秦川,等.石油烃类污染对青藏高原北麓河地区冻区土壤微生物多样性的影响[J].环境工程学报,2018,12(10):2917-2928.

浏览量

960

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

生物质炭保水剂的吸水保水性能研究

生物质炭对旱地红壤理化性状和水力学特性的影响

添加生物质炭对旱地红壤中硝态氮水平运移的影响

牧草品种与生物质炭对植草土壤水分的调控效应

青海省2009—2023年农业面源污染结构及变化特征