青藏高原高寒草甸退化对土壤氮素转化微生物基因的影响

王向涛; 张超; 廖李荣; 王杰; 喻岚辉; 张宪洲

doi:10.13961/j.cnki.stbctb.2020.03.002

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青藏高原高寒草甸退化对土壤氮素转化微生物基因的影响

试验研究

- 青藏高原高寒草甸退化对土壤氮素转化微生物基因的影响
- Effects of Degradation of Alpine Meadow on Soil Microbial Genes in Nitrogen Transformation in Qinghai-Tibet Plateau
- 水土保持通报 2020年40卷第3期页码：8-13
- 作者机构：
  
  1. 西藏农牧学院动物科学学院,西藏,林芝,860000
  2. 中国科学院地理科学与资源研究所, 生态系统网络观测与模拟重点实验室, 拉萨高原生态试验站,北京,100101
  3. 中国科学院大学,北京,100049
  4. 西北农林科技大学水土保持研究所, 陕西杨凌,712100
  5. 中国科学院大学资源与环境学院,北京,100190
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目“藏北高寒草地土壤种子库与植物多样性的关联及对草地恢复的作用机制”（41761008）;国家重点研发计划“西藏退化高寒生态系统恢复与重建技术及示范”（2016YFC0502001）
- DOI：10.13961/j.cnki.stbctb.2020.03.002
  中图分类号： S812.2
- 纸质出版：2020
- 稿件说明：
移动端阅览
王向涛, 张超, 廖李荣, 等. 青藏高原高寒草甸退化对土壤氮素转化微生物基因的影响[J]. 水土保持通报, 2020,40(3):8-13.

Wang Xiangtao, Zhang Chao, Liao Lirong, et al. Effects of Degradation of Alpine Meadow on Soil Microbial Genes in Nitrogen Transformation in Qinghai-Tibet Plateau[J]. Bulletin of Soiland Water Conservation, 2020, 40(3): 8-13.
王向涛, 张超, 廖李荣, 等. 青藏高原高寒草甸退化对土壤氮素转化微生物基因的影响[J]. 水土保持通报, 2020,40(3):8-13. DOI： 10.13961/j.cnki.stbctb.2020.03.002.

Wang Xiangtao, Zhang Chao, Liao Lirong, et al. Effects of Degradation of Alpine Meadow on Soil Microbial Genes in Nitrogen Transformation in Qinghai-Tibet Plateau[J]. Bulletin of Soiland Water Conservation, 2020, 40(3): 8-13. DOI： 10.13961/j.cnki.stbctb.2020.03.002.

摘要

[目的] 深入分析高寒草甸退化过程中土壤氮素转化特征，明确草甸退化对土壤氮素转化微生物基因丰度的影响，为认识高寒草甸的退化机理以及科学治理高寒退化草甸提供重要依据。[方法] 以青藏高原不同退化程度高寒草甸（未退化、轻度退化、中度退化、重度退化）为研究对象，利用实时定量PCR法分析退化过程中土壤理化性质及与氮素转化相关基因（nifH，amoA-AOA，amoA-AOB，narG，nirK，nirS和nosZ）丰度的变化，明确影响高寒草甸氮素转化基因的关键因子。[结果] ①随退化程度的加剧，高寒草甸土壤有机碳、全氮、硝态氮及铵态氮含量逐渐降低；②高寒草甸退化降低了与氮素转化相关的固氮nifH基因、氨氧化amoA-AOA和amoA-AOB基因丰度，但增加了反硝化narG，nirS和nirK基因丰度，且在重度退化草甸丰度最高；③nifH，amoA-AOA和amoA-AOB基因与土壤有机碳、硝态氮、铵态氮及水分呈显著正相关，narG，nirS和nirK基因与土壤有机碳、硝态氮及铵态氮含量呈显著负相关，与pH值呈显著正相关。[结论] 高寒草甸退化对氮素转化微生物具有重要影响，土壤有机碳、pH值及水分是影响土壤氮素转化微生物基因的主要因素。

Abstract

[Objective] The characteristics of nitrogen (N) transformation in degraded alpine meadows and identify the effects of meadow degradation on the gene abundance of soil nitrogen-transforming microorganisms were studied thoroughly

in order to provide a basis for understanding the degradation mechanism and scientific management of alpine meadows.[Methods] A soil survey along a degraded gradient

including undegraded

lightly degraded

moderately degraded

and severely degraded meadows

in the Qinghai-Tibetan alpine region was conducted. The changes in soil physicochemical properties and genes in N transformation (including nifH

amoA-AOA

amoA-AOB

narG

nirK

nirS

and nosZ) were investigated by using real-time quantitative PCR method

and the drivers of these functional genes were clarified.[Results] ① The contents of soil organic carbon (SOC)

total N

nitrate N

and ammonium N decreased along the degradation gradient. ② Degradation led to a significant reduction in the abundance of nifH

amoA-AOA

and amoA-AOB genes

whereas it caused an increase in the abundance of narG

nirS

and nirK genes

which was the maximum in the severely degraded meadow soils. ③ The abundance of the nifH

amoA-AOA

and amoA-AOB genes was significantly positively correlated with the contents of SOC

nitrate N

ammonium N

and soil moisture. The abundance of the narG

nirS

and nirK genes was significantly negatively correlated with the contents of SOC

nitrate N

and ammonium N

whereas it was positively correlated with the pH value.[Conclusion] The degradation of the alpine meadows had an important influence on the microorganisms involved in N transformation. The SOC

pH value

and moisture were the significant drivers of the functional genes in soil nitrogen-transforming microorganisms in alpine meadow soils.

关键词

Keywords

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