砒砂岩中植物促生芽孢杆菌的筛选及其对土壤的改良作用

邓琳; 王涛; 殷涂童; 何琳燕; 杨才千; 梁止水

doi:10.13961/j.cnki.stbctb.2019.05.029

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砒砂岩中植物促生芽孢杆菌的筛选及其对土壤的改良作用

试验研究

- 砒砂岩中植物促生芽孢杆菌的筛选及其对土壤的改良作用
- Isolation of Plant Growth Promoting Bacillus Spp. and Their Impact on Feldspathic Sandstone Soil Characteristics
- 水土保持通报 2019年39卷第5期页码：211-217
- 作者机构：
  
  1. 东南大学土木工程学院,江苏,南京,211189
  2. 农业部农业环境微生物重点实验室南京农业大学生命科学学院,江苏,南京,210095
- 作者简介：
- 基金信息：
  
  国家重点研发计划项目“砒砂岩区生态安全保障技术”（2017YFC0504505）;国家自然科学基金项目“卤代硝基甲烷在紫外/氯共同消毒过程中生成与降解的特性及机制”（21677032）
- DOI：10.13961/j.cnki.stbctb.2019.05.029
  中图分类号： Q93;S156;S182
- 纸质出版：2019
- 稿件说明：
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邓琳, 王涛, 殷涂童, 等. 砒砂岩中植物促生芽孢杆菌的筛选及其对土壤的改良作用[J]. 水土保持通报, 2019,39(5):211-217.

Deng Lin, Wang Tao, Yin Tutong, et al. Isolation of Plant Growth Promoting Bacillus Spp. and Their Impact on Feldspathic Sandstone Soil Characteristics[J]. Bulletin of Soiland Water Conservation, 2019, 39(5): 211-217.
邓琳, 王涛, 殷涂童, 等. 砒砂岩中植物促生芽孢杆菌的筛选及其对土壤的改良作用[J]. 水土保持通报, 2019,39(5):211-217. DOI： 10.13961/j.cnki.stbctb.2019.05.029.

Deng Lin, Wang Tao, Yin Tutong, et al. Isolation of Plant Growth Promoting Bacillus Spp. and Their Impact on Feldspathic Sandstone Soil Characteristics[J]. Bulletin of Soiland Water Conservation, 2019, 39(5): 211-217. DOI： 10.13961/j.cnki.stbctb.2019.05.029.

摘要

[目的] 筛选砒砂岩土壤中植物促生芽孢杆菌，为微生物强化植物改良砒砂岩土壤的科学设想提供资源和技术基础。[方法] 以产生植物激素IAA（indole acetic acid）、铁载体和生物膜为筛选指标，从内蒙古砒砂岩区土壤和植物样品中筛选植物促生芽孢杆菌，采用盆栽试验探究植物促生芽孢杆菌改良砒砂岩土壤特性和促进苜蓿和黑麦草生长的作用。[结果] 筛选到的12株芽孢杆菌产生IAA、铁载体和生物膜的能力不同，分别属于Bacillus halotolerans，B.atrophaeus，B.siamensis和B.zhangzhouensis种群。与对照相比，B.halotolerans P75能够显著增加砒砂岩土壤的有机质含量（24.7%）、速效磷含量（11.9%）和速效钾含量（21.0%）等养分指标，每1 g土壤可培养细菌达到7.4 lg CFU，土壤蔗糖酶活性显著增强（58.8%）。接种B.halotolerans P75后，在砒砂岩土壤中生长的苜蓿和黑麦草干重增加22.3%~81.5%，[结论] 从生长于砒砂岩土壤的苜蓿根内筛选到B.halotolerans P75，可提高砒砂岩土壤肥力，促进苜蓿和黑麦草生长，具有强化植物改良砒砂岩土壤的潜力。

Abstract

[Object] The characteristics of the plant growth promoting Bacillus spp. in a feldspathic sandstone soil were investigated in order to provide resources and technical information for feldspathic sandstone soil improvement.[Methods] Bacillus spp. was isolated from plants and feldspathic sandstone soil in a region of Inner Mongolia. The bacterium were identified by 16S rDNA sequence analysis. The ability of Bacillus spp. for producing indole acetic acid (IAA)

siderophores

and biofilms were tested. Greenhouse experiments were conducted to evaluate the effect of Bacillus spp. on the quality of feldspathic sandstone soil

and on the growth of alfalfa (Medicogo sativa) and perennial ryegrass (Lolium perenne).[Results] Twelve strains of Bacillus spp. were screened and had different production of IAA

siderophores

and biofilms

which belong to B. halotolerans

B. atrophaeus

B. siamensis and B. zhangzhouensis. The isolated B. halotolerans P75 was able to significantly increase the content of soil organic matter (24.7%)

available phosphorus (11.9%)

and available potassium (21.0%) in the sandstone compared with the non-inoculated control. Soil invertase activity (58.8%) and the number of culturable bacteria (7.4 lg CFU in 1 g soil) also significantly increased after B. halotolerans P75 inoculation. Moreover

B. halotolerans P75 significantly increased the dry weight of alfalfa and perennial ryegrass grown on the feldspathic sandstone soil by 22.3%~81.5% in comparison to those of the control.[Conclusion] Our results suggest that the B. halotolerans P75

isolated from the alfalfa roots growing in feldspathic sandstone area

can increase the fertility of feldspathic sandstone soil and promote the growth of alfalfa and ryegrass

and has great potential for the phytoremediation and improvement of feldspathic sandstone soil.

关键词

Keywords

references

王愿昌,吴永红,寇权,等.砒砂岩分布范围界定与类型区划分[J].中国水土保持科学,2007,5(1):14-18.

何京丽,殷丽强,郭建英,等.砒砂岩地区沙棘生态工程的土壤修复效果分析[J]. 国际沙棘研究与开发,2013,11(3):19-23.

Yang Fangshe, Cao Mingming, Li Huaien, et al. Simulation of sediment retention effects of the single seabuckthorn flexible dam in the Pisha Sandstone area[J]. Ecological Engineering, 2013,52(3):228-237.

肖培青,姚文艺,刘慧.砒砂岩地区水土流失研究进展与治理途径[J].人民黄河,2014,36(10):92-94,109.

毕慈芬,徐双民,李桂芬.砒砂岩地区沟道沙棘植物"柔性坝"原型拦沙研究[J].国际沙棘研究与开发,2003,1(1):6-12.

肖培青,姚文艺,申震洲,等.黄河中游砒砂岩区抗蚀促生技术研究[J].中国水土保持,2016(9):73-75.

姚文艺,刘慧,梁止水.砒砂岩区生态恢复的新途径:一种环境友好型抗蚀促生技术[J]. 水土保持通报,2018,38(4):118-124.

刘丹丹,李敏,刘润进.我国植物根围促生细菌研究进展[J]. 生态学杂志,2016,35(3):815-824.

Burd G I, Dixon D G, Glick B R. Plant growth-promoting bacteria that decrease heavy metal toxicity in plants[J]. Canadian Journal of Microbiology, 2000,46(3):237-245.

Yang Jungwook, Kloepper J W, Ryu C M. Rhizosphere bacteria help plants tolerate abiotic stress[J]. Trends in Plant Science, 2009,14(1):1-4.

余劲聪,何舒雅,曾润颖,等.芽孢杆菌修复土壤重金属镉污染的研究进展[J].广东农业科学,2016,43(1):73-78.

杨丽,燕传明,贺卓,等.重金属耐性芽孢杆菌的筛选及其对辣椒吸收镉铅的阻控效应[J].农业环境科学学报,2018,37(6):1086-1093.

庞海东,贺卓,燕传明,等.耐重金属的植物促生芽孢杆菌筛选及其强化香蒲去除Cd的作用[J].农业环境科学学报,2017,36(11):2314-2321.

Gordon S A, Weber R P. Colorimetric estimation of indoleacetic acid[J]. Plant Physiology, 1951,26(1):192-195.

Schwyn B, Neilands J B. Universal chemical assay for the detection and determination of siderophores[J]. Analytic Biochemistry, 1987,160(1):47-56.

Xi Jun, He Linyan, Huang Zhi, et al.

Bacillus qingshengii

sp. nov., a rock-weathering bacterium isolated from weathered rock surface[J]. International Journal of Systematic & Evolutionary Microbiology, 2014,64:2473-2479.

关松荫.土壤酶及其研究法[M]. 北京:农业出版社,1986:274-276,294-297.

鲍士旦.土壤农化分析[M].3版.北京:中国农业出版社,2000.

Vlamakis H, Chai Yunrong, Beauregard P, et al. Sticking together:building a biofilm the

Bacillus subtilis

way[J]. Nature Reviews Microbiology, 2013,11(3):157-168.

Xu Zhihui, Zhang Ruifu, Wang Dandan, et al. Enhanced control of cucumber wilt disease by

Bacillus amyloliquefaciens

SQR9 by altering the regulation of its DegU phosphorylation[J]. Applied & Environmental Microbiology, 2014, 80(9):2941-50.

徐文静,靳晓东,杨秋生.植物根际微生物的影响因素研究进展[J].河南农业科学,2014,43(5):6-12.

王浩,杨方社,李怀恩,等.沙棘柔性坝对砒砂岩沟道泥沙粒径分布及有机质影响[J].水土保持学报,2017,31(5):158-163.

卢立娜,赵雨兴,胡莉芳,等.沙棘(

Hippophae rhamnoides

)种植对鄂尔多斯砒砂岩地区土壤容重、孔隙度与贮水能力的影响[J]. 中国沙漠,2015,35(5):1171-1176.

刘龙,姚云峰,郭月峰,等.准格尔旗砒砂岩区三种典型造林树种蒸腾耗水研究[J].中国农业科技导报,2018,20(3):124-131.

梁止水,吴智仁,杨才千,等.基于W-OH的砒砂岩抗蚀促生机理研究[J].水利学报,2016,47(9):1160-1166.

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