覆盖和埋置白三叶对黄土高原苹果园土壤细菌多样性的影响

马震珠; 魏倩倩; 李尚玮; 杨文权; 寇建村

doi:10.13961/j.cnki.stbctb.2019.05.020

您当前的位置：

首页 >

文章列表页 >

覆盖和埋置白三叶对黄土高原苹果园土壤细菌多样性的影响

试验研究

- 覆盖和埋置白三叶对黄土高原苹果园土壤细菌多样性的影响
- Effects of Covering or Burying Trifolium Repens on Diversity of Soil Bacteria Community in an Apple Orchard in Loess Plateau
- 水土保持通报 2019年39卷第5期页码：143-151
- 作者机构：
  
  1. 西北农林科技大学草业与草原学院, 陕西杨凌,712100
  2. 西北农林科技大学生命科学学院, 陕西杨凌,712100
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目“黄土高原水热季节耦合对苹果园白三叶降解形成土壤有机质的影响机理”（31302027）
- DOI：10.13961/j.cnki.stbctb.2019.05.020
  中图分类号： S182;S154.3
- 纸质出版：2019
- 稿件说明：
移动端阅览
马震珠, 魏倩倩, 李尚玮, 等. 覆盖和埋置白三叶对黄土高原苹果园土壤细菌多样性的影响[J]. 水土保持通报, 2019,39(5):143-151.

Ma Zhenzhu, Wei Qianqian, Li Shangwei, et al. Effects of Covering or Burying Trifolium Repens on Diversity of Soil Bacteria Community in an Apple Orchard in Loess Plateau[J]. Bulletin of Soiland Water Conservation, 2019, 39(5): 143-151.
马震珠, 魏倩倩, 李尚玮, 等. 覆盖和埋置白三叶对黄土高原苹果园土壤细菌多样性的影响[J]. 水土保持通报, 2019,39(5):143-151. DOI： 10.13961/j.cnki.stbctb.2019.05.020.

Ma Zhenzhu, Wei Qianqian, Li Shangwei, et al. Effects of Covering or Burying Trifolium Repens on Diversity of Soil Bacteria Community in an Apple Orchard in Loess Plateau[J]. Bulletin of Soiland Water Conservation, 2019, 39(5): 143-151. DOI： 10.13961/j.cnki.stbctb.2019.05.020.

摘要

[目的] 探究白三叶在不同降解时期果园土壤细菌多样性的变化，为果园白三叶的利用及果园生草模式的推广提供理论依据。[方法] 在陕西省洛川县苹果园内分别以覆盖和埋置两种方式将白三叶（Trifolium repens）返园，通过高通量测序研究两种返园方式下白三叶降解1，3，6，12个月时土壤细菌的多样性、丰度及群落结构。[结果] ①白三叶返园处理下，土壤微生物优势菌群种类未表现出显著变化，丰度存在差异，返园处理组土壤中出现梭杆菌门（Acidobacteria）、广古菌门（Euryarchaeota）、泉古菌门（Crenarchaeota）、螺旋体门（Spirochaetes）和硝化螺旋菌（Nitrospira）门等特有菌群。②白三叶返园对苹果园土壤细菌群落分布存在显著影响（p<0.05），且不同降解时期存在差异。两种返园处理组中酸杆菌门（Acidobacteria）、拟杆菌门（Bacteroidetes）、变形菌门（Proteobacteria）丰度增高，厚壁菌门（Firmicutes）丰度降低。③随着白三叶降解时间增加，芽单胞菌（Gemmatimonas）、黄杆菌（Flavobacterium）、Opitutu和Arenimonas属的细菌相对丰度增高，硝化螺旋菌属（Nitrospira）、奈瑟菌属（Neisseria）、Pirellula和Steroidobacter的丰度降低。[结论] 覆盖和埋置白三叶均能改善土壤菌群结构，提高有益菌丰度，促进土壤微生物生态系统物质循环。

Abstract

[Objective] The return of Trifolium repens to an apple orchard was investigated to understand the effects on soil bacteria diversity in different degradation stages

and to provide theoretical basis for the utilization of T. repens in orchards and the promotion of pasture-planting in orchards.[Methods] Fresh T. repens was covered or buried in an apple orchard in Luochuan County

Shannxi Province. At 1

6 and 12 months of T. repens degradation

soil samples were collected

and high-throughput sequencing technology was performed to determine the diversity

richness and structure of soil bacteria.[Results] ① There were no significant differences in the diversity of preponderant bacteria after T. repens returning

but some richness was different. Acidobacteria

Euryarchaeota

Crenarchaeota

Spirochaetes and Nitrospira were found in the soil of the return treatment group. ② There were some remarkable differences in community distribution of soil bacteria (p<0.05)

varying in different degrading period after the return of T. repens. The richness of Acidobacteria

Bacteroidetes and Proteobacteria increased

but that of Firmicutes decreased in soil samples from the two treatment groups compared with the control. ③ Over time

the richness of Gemmatimonas

Flavobacterium

Opitutu

and Arenimonas increased in groups in which T. repens had returned; however

the richness of Nitrospira

Neisseria

Pirellula

and Steroidobacter decreased.[Conclusion] Either covering or burying T. repens could optimize structure of soil bacteria community

increase probiotics abundance and improve the matter cycle in the microbial ecosystem.

关键词

Keywords

references

Food and Agriculture Organization of the United Nations Statistics Division, FAO statistical database[EB/OL].(2019-3-24)[2019-4-04] http://www.fao.org/faostat/en/#data/QL.

于林霞,张波,白秀广.黄土高原区苹果生产技术效率及其影响因素研究:基于528户苹果种植户的调查数据[J].干旱区资源与环境,2018,32(4):68-74.

孙馨宇,张枭,张鹏,等.温度、水分及有机物料对苹果园土壤有机碳转化和微生物群落多样性的影响[J].土壤通报,2018,49(4):822-833.

Guo J H, Liu X J, Zhang Y, et al. Significant acidification in major Chinese croplands[J]. Science, 2010, 327(5968):1008-1010.

Liu Xuejun, Zhang Ying, Han Wenxuan, et al. Enhanced nitrogen deposition over China[J].Nature, 2013,494(7438):459-462.

寇建村,杨文权,韩明玉,等.我国果园生草研究进展[J].草业科学,2010,27(7):154-159.

李发林,郑域茹,郑涛,等.果园带状生草对果园面源污染的控制效果[J].水土保持学报,2013,27(3):82-89.

Qian Xun, Gu Jie, Pan Hongjia, et al. Effects of living mulches on the soil nutrient contents, enzyme activities, and bacterial community diversities of apple orchard soils[J]. European Journal of Soil Biology, 2015,70:23-30.

van der Heijden M G A, Wagg C. Soil microbial diversity and agro-ecosystem functioning[J]. Plant and Soil, 2013,363(1/2):1-5.

王岳坤,洪葵.红树林土壤细菌群落16S rDNA V3片段PCR产物的DGGE分析[J].微生物学报,2005,45(2):201-204.

Kulmatiski A, Beard K H, Stevens J R, et al. Plant-soil feedbacks:A meta-analytical review[J]. Ecology Letters, 2008,11(9):980-992.

Tan B, Ng C, Nshimyimana J P, et al. Next-generation sequencing (NGS) for assessment of microbial water quality:Current progress, challenges, and future opportunities[J].Frontiers in Microbiology, 2015,6:1-20.

立天宇,康峰峰,韩海荣,等.冀北辽河源自然保护区土壤微生物碳代谢对阔叶林叶凋落物组成的响应[J].应用生态学报,2015,26(3):715-722.

Sakala W D, Cadisch G, Giller K E. Interactions between residues of maize and pigeonpea and mineral N fertilizers during decomposition and N mineralization[J]. Soil Biology & Biochemistry, 2000,32(5):679-688.

Pitta D W, Pinchak W E, Dowd S E, et al. Rumen bacterial diversity dynamics associated with changing from bermudagrass hay to grazed winter wheat diets[J]. Microbial Ecology, 2010,59(3):511-522.

Shannon C E. A mathematical theory of communication[J]. ACM SIGMOBILE Mobile Computing and Communications Review, 2001,5(1):3-55.

刘富庭.覆盖或生草措施对黄土高原地区苹果园土壤有机碳组分及微生物的影响[D]. 陕西杨凌:西北农林科技大学,2014.

李昌明,王晓玥,孙波.基于固态

C核磁共振波谱研究植物残体分解和转化机制的进展[J].土壤,2017,49(4):658-664.

Aerts R. The freezer defrosting:Global warming and litter decomposition rates in cold biomes[J]. Journal of Ecology, 2006,94(4):713-724.

Kogel-Knabner I. The macromolecular organic composition of plant and microbial residues as inputs to soil organic matter[J]. Cheminform, 2010,33(23):139-162.

Berg B, McClaugherty C. Initial litter chemical composition[M]//Plant Litter. Berlin, Heidelberg:Springer Berlin Heidelberg, 2003:49-77.

Janssen P H, Yates P S, Grinton B E, et al. Improved culturability of soil bacteria and isolation in pure culture of novel members of the divisions acidobacteria, actinobacteria, proteobacteria, and verrucomicrobia[J]. Applied and Environmental Microbiology, 2002,68(5):2391-2396.

Pankratov T A, Kirsanova L A, Kaparullina E N, et al. Telmatobacter bradus gen. nov. sp. nov. a cellulolytic facultative anaerobe from subdivision 1 of the Acidobacteria, and emended description of Acidobacterium capsulatum Kishimoto et al. 1991[J]. International Journal of Systematic & Evolutionary Microbiology, 2012,62(2):430-437.

Rawat S R, Mannisto M K, Yana B, et al. Comparative genomic and physiological analysis provides insights into the role of Acidobacteria in organic carbon utilization in Arctic tundra soils[J]. Fems Microbiol Ecology, 2012, 82(2):341-355.

Nguyen H T T, Le V Q, Hansen A A, et al. High diversity and abundance of putative polyphosphate-accumulating Tetrasphaera-related bacteria in activated sludge systems[J]. Fems microbiology ecology 2011,76(2):256-267.

张明锦,张健,纪托未,等.林窗对凋落物分解过程中细菌群落结构和多样性的影响[J].生态环境学报,2015,24(8):1287-1294.

王晓玥,孙波.植物残体分解过程中微生物群落变化影响因素研究进展[J].土壤,2012,44(3):353-359.

García-Orenes F, Morugán-Coronado A, Zornoza R, et al. Changes in soil microbial community structure influenced by agricultural management practices in a Mediterranean agro-ecosystem[J]. PloS One, 2013,8(11):e80522.

焦奎宝.生草制苹果园土壤微生物群落结构与功能特征研究[D].沈阳:沈阳农业大学,2014

刘晶,谢婉余,张巧明,等.黄土丘陵区不同植物凋落叶片的分解及养分释放特性[J].草业学报,2018,27(9):27-35.

浏览量

1072

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

黄土高原坡地苹果园土壤肥力及矿质氮累积分析

洛川县不同种植年限果园土壤基本性质与重金属含量评价

污泥有机肥改良铅锌尾矿对4种草本植物生长和重金属积累的影响

水分对苹果园土壤呼吸季节性与年际变化的影响

不同空间尺度耕地埂坎提取方法及其分布特征