油污土壤修复微生物的筛选及其影响因素

慕庆峰; 于立红; 张涛; 贾洪柏; 吴慧云

doi:10.13961/j.cnki.stbctb.2018.05.052

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油污土壤修复微生物的筛选及其影响因素

试验研究

- 油污土壤修复微生物的筛选及其影响因素
- Screening of Microorganisms for Remediation of Oil-contaminated Soil and Its Influencing Factors
- 水土保持通报 2018年38卷第5期页码：330-335
- 作者机构：
  
  1. 黑龙江八一农垦大学农学院寒地作物栽培技术省级重点实验室,黑龙江,大庆,163319
  2. 中海油能源发展股份有限公司工程技术分公司,天津,300452
  3. 东北林业大学, 生命科学学院,黑龙江,哈尔滨,150040
- 作者简介：
- 基金信息：
  
  大庆油田企业合作项目“石油污染土壤生物原位修复技术应用研究”（DQYT-0508003-2013-JS-S7S）
- DOI：10.13961/j.cnki.stbctb.2018.05.052
  中图分类号： X53
- 纸质出版：2018
- 稿件说明：
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慕庆峰, 于立红, 张涛, 等. 油污土壤修复微生物的筛选及其影响因素[J]. 水土保持通报, 2018,38(5):330-335.

MU Qingfeng, YU Lihong, ZHANG Tao, et al. Screening of Microorganisms for Remediation of Oil-contaminated Soil and Its Influencing Factors[J]. Bulletin of Soiland Water Conservation, 2018, 38(5): 330-335.
慕庆峰, 于立红, 张涛, 等. 油污土壤修复微生物的筛选及其影响因素[J]. 水土保持通报, 2018,38(5):330-335. DOI： 10.13961/j.cnki.stbctb.2018.05.052.

MU Qingfeng, YU Lihong, ZHANG Tao, et al. Screening of Microorganisms for Remediation of Oil-contaminated Soil and Its Influencing Factors[J]. Bulletin of Soiland Water Conservation, 2018, 38(5): 330-335. DOI： 10.13961/j.cnki.stbctb.2018.05.052.

摘要

[目的

]

探讨微生物原位修复的主要影响因素及水平（正交设计）之间的关系，为石油污染场地生物修复工程的参数设计提供一定理论依据。[方法

]

选取5因素4水平的正交设计，考察污染强度、营养物、氧化剂、表活剂、接菌量等因素对土壤修复效果的影响。[结果

]

以原油为唯一碳源经过初步筛选，获得16株石油烃降解优势菌，经过菌群复筛，获得2株偏利共生协同真菌DPF

，DPF

，协同降解率最高，7 d达87.77%。选择其进行室内油污土壤的微生物修复模拟试验，60 d石油污染强度为10的油污土壤降解率最高，可达94.12%。污染强度为25的油污土壤降解率为90.17%，SPSS数据分析表明生物修复影响的最大影响因素是氧化剂、表活剂和营养物，其次是污染强度、接菌量。[结论

]

初期添加表活剂、氧化剂、营养剂能对石油生物修复具有重要意义。污染强度仅在35 d前有一定影响，在修复后期影响最小。在整个修复过程，接菌量方差均值与其他因素比较都最小，因此其因素水平对石油降解能力的影响不显著。

Abstract

[Objective] To reveal the relationship between the main influencing factors and the level (orthogonal design) of microbial in situ remediation in order to provide a theoretical basis for the parameter design of bio-remediation engineering in oil-contaminated sites.[Methods] The effects of pollution intensity

nutrition

oxidant

surface active agent and inoculation amount on soil remediation were investigated by orthogonal design with 5 factors and 4 levels in the experiment.[Results] After the preliminary screening of crude oil as the sole carbon source

16 dominant bacteria strains for petroleum hydrocarbon degradation were obtained. By the bacterial rescreening

two symbiotic fungi DPF2 and DPF4 were obtained. The highest synergistic degradation rate was 87.77% after 7 days. The degradation rate of oil-contaminated soil was the highest when the oil pollution intensity was 10 after 60 days

and the degradation rate of oil-contaminated soil reached to 94.12%. The degradation rate of oil-contaminated soil with pollution intensity of 25 was 90.17%. SPSS data analysis showed that oxidants

surfactant and nutrients were the most important factors affecting bio-remediation

followed by the intensity of contamination and the number of bacteria.[Conclusion] The initial addition of surfactant

oxidants

and nutrients could be of great significance to petroleum bio-remediation. The pollution intensity had a certain effect before 35 days

and had the least influence in the later stage of remediation. During the whole remediation process

the mean variance of inoculation amount was the smallest compared with other factors

so the factor level had no significant effect on the oil degradation ability.

关键词

Keywords

references

张宝良.油田土壤石油污染与原位生物修复技术研究[D].黑龙江大庆:大庆石油学院,2007:57-74.

陆秀君,郭书海,孙清,等.石油污染土壤的修复技术研究现状及展望[J].沈阳农业大学学报,2003,34(1):63-67.

Propst L T, Lochmiller L R, Qualls J C W, et al. In situ(mesocosm) assessment of immunotoxicity risks to small mammals inhabiting pet-rochemical waste sites[J]. Chemosphere, 1999,38(5):1049-1067.

刁潘.化学热洗-生物降解联合处理含油污泥[D].重庆:西南石油大学,2014:8-33.

Atlas R M. Microbial hydrocarbon degradation-bioremediation of oil spills[J]. Journal of Chemical Technology and Bio-technology, 1991,52(2):149-156.

李春荣,王文科,曹玉清,等.石油污染土壤的生物修复研究[J].农业环境科学学报2009,28(2):234-238.

张秀霞,白雪晶,徐娜娜,等.固定化微生物修复石油污染土壤影响因素研究[J].环境工程学报,2013,7(3):1156-1162.

黄廷林,唐智新,徐金兰,等.黄土地区石油污染土壤生物修复室内模拟试验研究[J].农业环境科学学报,2008,27(6):2206-2210.

ljah U J J, Antai S P. Removal of nigerian light crude oil in soil over a 12-month period[J]. International Biodeterioration & Biodegradation.2003,51(2):93-99.

Margesin R, Hmmerle M, Tscherko D. Microbial activity and community composition during bioremediation of diesel-oil-contaminated soil:Effects of hydrocarbon concentration, fertilizers, and incubation time[J]. Microbial Ecology, 2007,53(2):259-269.

张秀霞,滕芝,吴佳东,等.激活剂对石油污染土壤修复的强化作用及修复条件的优化[J].石油学报:石油加工,2013,29(2):353-358.

王冬梅,陈丽华,周立辉,等.鼠李糖脂对微生物菌剂降解石油的影响[J].环境工程学报,2013,10(7):4121-4126.

李燕妮.细菌菌群降解石油的影响因素及应用研究[D].天津:天津理工大学,2013:54-61.

Santisi S, Cappello S, Catalfamo M, et al. Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium[J]. Brazilian Journal of Microbiology, 2015,46(2):377-387.

Jianliang Xue, Yang Yu, Yu Bai, et al. Marine Oil-Degrading Microorganisms and Biodegradation Process of Petroleum Hydrocarbon in Marine Environments: A review[J]. Curr Microbiol, 2015,71:220-228.

张春华,严云良.医药数理统计[M].北京:科学出版社出版,2001.

乔俊,陈威.添加不同营养助剂对石油污染土壤生物修复的影响[J].环境化学,2010,29(1):6-11.

Jamgocian R P, Yapijakis C. Pilot-scale comparison of bioventing vs. hydrogen peroxide in maintaining effective aerobic in situ, bioremediation[J]. Studies in Environmental Science, 1997,66:365-377.

Merkova M, Zalesak M, Ringlova E, et al. Degradation of the surfactant Cocamidopropyl betaine by two bacterial strains isolated from activated sludge[J]. International Biodeterioration & Biodegradation, 2018,127:236-240.

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