黑果枸杞种子萌发对PEG模拟干旱胁迫的响应

郭有燕; 余宏远; 孔东升; 张亚娟; 吴佳豫

doi:10.13961/j.cnki.stbctb.2017.05.017

您当前的位置：

首页 >

文章列表页 >

黑果枸杞种子萌发对PEG模拟干旱胁迫的响应

试验研究

- 黑果枸杞种子萌发对PEG模拟干旱胁迫的响应
- Response of Seed Germination of Lycium Ruthenicum to PEG-Simulated Drought Stress
- 水土保持通报 2017年37卷第5期页码：98-102
- 作者机构：
  
  1. 河西学院河西学院黑果枸杞工程中心,甘肃,张掖,734000
  2. 河西学院甘肃省河西走廊特色资源利用重点实验室,甘肃,张掖,734000
  3. 甘肃农业大学林学院,甘肃,兰州,730070
- 作者简介：
- 基金信息：
  
  国家自然科学基金地区项目“荒漠药用植物黑果枸杞实生苗更新过程的生态学研究”（31460189），“黑果枸杞无性成苗的生态过程及生殖选择研究”（31660193）;甘肃省高等学校科研项目“荒漠药用植物黑果枸杞对干旱胁迫的适应能力和适应机制研究项目”（2014A-111）
- DOI：10.13961/j.cnki.stbctb.2017.05.017
  中图分类号：
- 纸质出版：2017
- 稿件说明：
移动端阅览
郭有燕, 余宏远, 孔东升, 等. 黑果枸杞种子萌发对PEG模拟干旱胁迫的响应[J]. 水土保持通报, 2017,37(5):98-102.

GUO Youyan, YU Hongyuan, KONG Dongsheng, et al. Response of Seed Germination of Lycium Ruthenicum to PEG-Simulated Drought Stress[J]. Bulletin of Soiland Water Conservation, 2017, 37(5): 98-102.
郭有燕, 余宏远, 孔东升, 等. 黑果枸杞种子萌发对PEG模拟干旱胁迫的响应[J]. 水土保持通报, 2017,37(5):98-102. DOI： 10.13961/j.cnki.stbctb.2017.05.017.

GUO Youyan, YU Hongyuan, KONG Dongsheng, et al. Response of Seed Germination of Lycium Ruthenicum to PEG-Simulated Drought Stress[J]. Bulletin of Soiland Water Conservation, 2017, 37(5): 98-102. DOI： 10.13961/j.cnki.stbctb.2017.05.017.

摘要

[目的] 研究干旱胁迫对黑果枸杞种子萌发及幼苗生长的影响，为黑果枸杞人工栽培提供理论依据。[方法] 通过聚乙二醇（polyethylene glycol，PEG）模拟干旱胁迫，测定不同干旱胁迫强度下黑果枸杞种子的萌发进程、发芽率、发芽势、发芽指数、活力指数、幼苗的生长、幼苗渗透调节物质、叶绿素含量等指标，研究干旱胁迫对黑果枸杞种子萌发及幼苗生长的影响。[结果] 干旱胁迫不利于黑果枸杞种子的萌发，重度干旱胁迫推迟其萌发的高峰期；干旱胁迫下黑果枸杞种子的发芽率、发芽势均呈下降趋势，-0.6 MPa处理下，黑果枸杞种子发芽率、发芽势相对对照分别降低72.06%和74.07%；干旱胁迫对黑果枸杞种子发芽指数和活力指数有抑制作用，其抑制程度随干旱胁迫强度的增加而增加；适度干旱胁迫有利于黑果枸杞幼苗胚根和胚芽的生长，而中度、重度干旱胁迫不利于幼苗胚芽的生长；干旱胁迫下黑果枸杞幼苗根、芽脯氨酸含量和可溶性糖含量均随着胁迫程度的增加逐渐增加，而幼苗叶绿素含量（Chl a+b）随干旱胁迫强度的增加逐渐降低。[结论] 干旱胁迫对黑果枸杞种子萌发及幼苗生长有重要影响，适度的干旱胁迫有利于黑果枸杞幼苗的生长。

Abstract

[Objective] To study the response of seed germination and seedling growth of Lycium ruthenicum to PEG(polyethylene glycol)-simulated drought stress

and to provide a theoretical basis for artificial cultivation of L. ruthenicum.[Methods] PEG-6000 solution was used to simulate drought stress. Seed germination progress

germination percentage

germination potential

germination index

vital index

seedling growth

seedling osmotic adjustment substances

and chlorophyⅡ content under water stress were recorded. The effects of drought stress on seed germination and seedling growth of L. ruthenicum were studied.[Results] Drought stress was disadvantaged to seed germination of L. ruthenicum

and the severe drought stress will delay the fastigium of L. ruthenicum seed germination. Seed germination percentage and germination potential decreased with the increasing level of drought stress. Under -0.6 MPa treatment

germination percentage and germination potential decreased by 72.06% and 74.07% as compared to the one of control treatment. Germination index and vital index were restrained under drought stress

and the restraining degree increased with the increasing of drought stress. The moderate drought stress was beneficial to the growth of radicle and germ

however

it wasn't beneficial to the growth of germ under medium and severe drought stress. Proline and soluble sugar of seedling root and shoot increased with the increasing drought stress level. However

chlorophyⅡ content decreased with the increasing drought stress level.[Conclusion] Drought stress had important effect to the seed germination and seedling growth of L. ruthenicum

and the moderate drought stress was beneficial to the growth of L. ruthenicum seedling.

关键词

Keywords

references

Koochaki A. Farming plant germination physiological fundamentals[J]. Astane Ghodse Razavi Publication, 1991,98:869-874.

Donohue K, Dorn L, Griffith C, et al. The evolutionary ecology of seed germination of Arabidopsis thaliana:variable natural selection on germination timing[J]. Evolution, 2005,59(4):758-770.

Hardegree S P, Emmerich W E. Effect of polyethylene glycol exclusion on the water potential of solution-saturated filter paper[J]. Plant Physiology, 1990, 92(2):462-466.

Gorai M, Tlig T, Neffati M. Influence of water stress on seed germination characteristics in invasive

Diplotaxis harra

(Forssk.) Boiss (Brassicaceae) in arid zone of Tunisia[J]. Journal of Phytology, 2009,1(4):249-254.

Mantovani A, Iglesias R R. The effect of water stress on seed germination of three terrestrial bromeliads from restinga[J]. Brazilian Journal of Botany, 2010,33(1):201-205.

Naseri H. Investigation on botanical and ecological characteristics of

Artemisia

spices in east Azarbayjan[C]//M. Sc. seminar. Faculty of Natural Resources, University of Tehran, 2003,92.

郭有燕,孔东升,闫芳,等.干旱胁迫对黑果枸杞光合特性的影响[J].西北植物学报,2016,36(1):124-130.

冯建森,刘志虎.酒泉市野生资源黑果枸杞资源及利用[J].林业实用技术,2013(2):62-64.

Zheng Jie, Ding Chengxu, Wang Liangsheng, et al. Anthocyanins composition and antioxidant activity of wild

Lycium ruthenicum

Murr. from Qinghai-Tibet Plateau[J]. Food Chemistry, 2011,126(3):859-865.

Liu Zenggen, Dang Jun, Wang Qilian, et al. Optimization of polysaccharides from

Lycium ruthenicum

fruit using RSM and its anti-oxidant activity[J]. International journal of biological macromolecules, 2013, 61:127-134.

Liu Zenggen, Shu Qingyan, Wang Lei, et al. Genetic diversity of the endangered and medically important

Lycium ruthenicum

Murr. revealed by sequence-related amplified polymorphism(SRAP)markers[J]. Biochemical Systema tics and Ecology,2012,45:86-97.

王锦楠,陈进福,陈武生,等.柴达木地区野生黑果枸杞种群遗传多样性的AFLP分析[J].植物生态学报,2015,39(10):1003-1011.

Chen Haikui, Zhao Wenhong. Effects of NaCl stress on seed germination of

Lycium ruthenicum

Murr[J]. Agricultural Science & Technology-Hunan, 2010,11(4):37-38.

王桔红,陈文.黑果枸杞种子萌发及幼苗生长对盐胁迫的响应[J].生态学杂志,2012,31(4):804-810.

韩多红,李善家,王恩军,等.外源钙对盐胁迫下黑果枸杞种子萌发和幼苗生理特性的影响[J].中国中药杂志,2014,29(1):34-39.

王恩军,李善家,韩多红,等.中性盐和碱性盐胁迫对黑果枸杞种子萌发及幼苗生长的影响[J].干旱地区农业研究,2014,32(6):64-69.

Michel B E, Kaufmann M R. The osmotic potential of polyethylene glycol6000[J]. Plant Physiology,1973,51(5):914-916.

李合生.植物生理生化试验原理与技术[M].北京:高等教育出版社,2000.

张志良,瞿伟菁.植物生理学试验指导[M].北京:高等教育出版社,2003.

张勇,薛林贵,高天鹏,等.荒漠植物种子萌发研究进展[J].中国沙漠,2005,25(1):106-112.

齐淑艳,段继鹏,郭婷婷,等.入侵植物牛膝菊种子萌发对PEG模拟干旱胁迫的响应[J].生态学杂志,2014,33(5):1190-1194.

刘自刚,沈冰,张雁.桔梗种子萌发对低温、干旱及互作胁迫的响应[J].生态学报,2013,33(8):2615-2622.

何芳兰,赵明,王继和,等.几种荒漠植物种子萌发对干旱胁迫的响应及其抗旱性评价研究[J].干旱区地理,2011,34(1):100-106.

杨景宁,王彦荣. PEG模拟干旱胁迫对四种荒漠植物种子萌发的影响[J].草业学报,2012,21(6):23-29.

秦文静,梁宗锁.四种豆科牧草萌发期对干旱胁迫的响应及抗旱性评价[J].草业学报,2010,19(4):61-70.

Gratani L, Varone L. Leaf key traits of Erica arborea L., Erica multiflora L. and Rosmarinus officinalis L. co-occurring in the Mediterranean maquis[J]. Flora-Morphology, Distribution, Functional Ecology of Plants, 2004,199(1):58-69.

朱志梅,杨持.草原沙漠化过程中植物的耐胁迫类型研究[J].生态学报,2004,24(6):1093-1100.

浏览量

1935

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

干旱胁迫对窄叶鲜卑花种子萌发及幼苗生长的影响

不同种源长柄扁桃种子萌发期耐旱及耐盐性

高压电场对毛乌素沙地两种优势植物抗旱性的影响

黑麦草对土壤中Pb的富积作用及耐受性研究

聚乙二醇和低温胁迫对红豆草种子萌发影响的研究