1. 滨州学院 资源与环境工程学院,山东,滨州,256600
2. 滨州学院 生物工程学院系,山东,滨州,256600
纸质出版:2017
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李学平, 谢文军, 范延辉. 盐碱地塔宾曲霉菌的解磷能力及其对小麦生长的影响[J]. 水土保持通报, 2017,37(1):93-96.
LI Xueping, XIE Wenjun, FAN Yanhui. Phosphate-solubilizing Ability of Aspergillus Tubingensis and Its Effects on Growth of Wheat in Seedling Stage[J]. Bulletin of Soiland Water Conservation, 2017, 37(1): 93-96.
李学平, 谢文军, 范延辉. 盐碱地塔宾曲霉菌的解磷能力及其对小麦生长的影响[J]. 水土保持通报, 2017,37(1):93-96. DOI: 10.13961/j.cnki.stbctb.2017.01.017.
LI Xueping, XIE Wenjun, FAN Yanhui. Phosphate-solubilizing Ability of Aspergillus Tubingensis and Its Effects on Growth of Wheat in Seedling Stage[J]. Bulletin of Soiland Water Conservation, 2017, 37(1): 93-96. DOI: 10.13961/j.cnki.stbctb.2017.01.017.
[目的
]
研发新型解磷生物菌肥,提高黄河三角洲盐碱障碍耕地作物产量。[方法
]
采用无机磷液体培养基培养的方法,从黄河三角洲盐碱化菜园根际土壤筛选得到一株解磷真菌CT
1
,即塔滨曲霉菌(
Aspergillus tubingensis
),对其解磷能力进行了深入研究。[结果
]
解磷菌CT
1
的解磷能力随发酵液盐浓度升高降低,当发酵液盐浓度在0.03%~6%时,发酵液中有效磷浓度可维持在523.5~338.5 mg/L,且解磷菌的溶磷量与发酵液pH之间存在明显的负相关。解磷菌CT
1
在葡萄糖作为碳源的培养基上生长状况最好,在(NH
4
)
2
SO
4
作为氮源的培养基上生长状况最好。接入解磷菌15 d的小麦与未接菌的小麦相比,茎长增加了16.24%,茎鲜重增加了12.35%,根长增加了21.6%。[结论
]
解磷菌CT
1
对盐碱地小麦幼苗生长有一定的促进作用,可作为提高盐碱地作物产量的新型解磷生物菌肥利用。
[Objective] The objective of the study is to research a new biological phosphorus fertilizer in order to improve crop yield in alkaline soil in the Yellow River Delta. [Methods] The cultivating method used in this study was inorganic phosphorus liquid culture medium. Fungi Aspergillus tubingensis(CT1) was screened from the rhizosphere soil in the garden of Yellow River delta. We researched the phosphate-solubilizing ability of fungi. [Results] The phosphate-solubilizing ability of fungi CT1 was reduced with the increasing of fermented liquid salt concentration. When the concentration of NaCl was between 0.03% and 6%
the concentration of available phosphorus was maintained between 523.5 and 338.5 mg/L. There was an obvious correlation between the available phosphorus concentration and fermented liquid pH vale. CT1 could grow normally under four different kinds of carbon sources
and the glucose was the best culture medium. CT1 could grow normally under four different kinds of nitrogen source
and (NH4)2 SO4 was the best medium. Compared with uninoculated wheat
fungi CT1 inoculated wheat
after 15 days of wheat cultivation
the length of the stem increased by 16.24%
the fresh weight of the stem increased by 12.35%
and the root length increased by 21.6%. [Conclusion] Phosphate solubilizing bacteria CT1 could promote crop yield of saline-alkali soil
and can be used as a new phosphate-solubilizing fertilizer.
刘文干.三株红壤高效溶磷菌的分离、鉴定、溶磷特性及其对花生促生效应的研究[D].南京:南京农业大学,2012.
王莉晶.高效解磷菌的筛选及其对小麦生长的影响[D].辽宁大连:大连理工大学,2008.
贺梦醒,高毅,胡正雪,等.解磷菌株B25的筛选、鉴定及其解磷能力[J].应用生态学报,2012,23(1):235-239.
龚明波.溶磷微生物分离、应用及其相关基因的克隆与功能鉴定[D].北京:中国农业科学院,2011.
Kucey R. Phosphate solubilizing bacteria and fungi in various cultivated and virgin Alberta soils[J]. Canadian Journal of Soil Science, 1983,63(4):671-678.
刘文干,曹慧,樊建波,等.一株红壤花生根际溶磷真菌的分离、鉴定及溶磷能力的研究[J].土壤学报,2012,49(5):988-995.
艾力·吐热克.草酸青霉菌(P-O-41)的解磷与诱导抗性的研究[D].乌鲁木齐:新疆农业大学,2006.
段秀梅,高晓蓉,吕军,等.两株土壤分离菌的解磷能力及对玉米的促生作用[J].中国土壤与肥料,2010,6(2):79-85.
朱培淼,杨兴明,徐阳春,等.高效解磷细菌的筛选及其对玉米苗期生长的促进作用[J].应用生态学报,2007,18(1):107-112.
Babana A H, Antoun H. Biological system for improving the availability of Tilemsi phosphate rock for wheat(
Triticum aestivum
L
.
) cultivated in Mali[J]. Nutrient Cycling in Agroeco-systems, 2005,72(2):147-157.
Hameeda B, Srijana M, Rupela O P. Effect of bacteria isolated from composts and macrofauna on sorghum growth and mycorrhizal colonization[J]. World Journal of Microbiology Biotechnology, 2007,23:883-887.
刘长霞,谭天伟,翟洪杰.盐碱条件对真菌解磷能力的影响[J].微生物学通报,2002,30(5):69-72.
鲍士旦,土壤农化分析[M].北京:中国农业出版社,1999.
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