石油烃污染对陇东黄土高原土壤生物学及非生物学特性的影响

王金成; 井明博; 段春燕; 王国锋; 石国玺; 周天林

doi:10.13961/j.cnki.stbctb.2017.01.002

您当前的位置：

首页 >

文章列表页 >

石油烃污染对陇东黄土高原土壤生物学及非生物学特性的影响

试验研究

- 石油烃污染对陇东黄土高原土壤生物学及非生物学特性的影响
- Impact of Crude Oil Pollution on Soil Biological and Abiological Properties in Eastern Gansu Province
- 水土保持通报 2017年37卷第1期页码：9-16
- 作者机构：
  
  1. 甘肃省高校陇东生物资源保护与利用省级重点实验室,甘肃,庆阳,745000
  2. 陇东学院生命科学与技术学院,甘肃,庆阳,745000
  3. 陕西师范大学生命科学学院,陕西,西安,710062
  4. 中国科学院西北高原生物研究所,青海,西宁,810008
- 作者简介：
- 基金信息：
  
  甘肃省科技支撑计划项目“陇东黄土高原地区石油污染土壤原位修复技术研究与示范”（1204FKCM173）;甘肃省教育厅科技计划项目“陇东黄土高原石油污染土壤微生物群落动态分析及石油降解菌的筛选”（2014B-091）;国家自然科学资助项目（31500427）;甘肃省庆阳市科技支撑计划项目（GC2011-16）;陇东学院青年科技创新项目（XYZK1603）
- DOI：10.13961/j.cnki.stbctb.2017.01.002
  中图分类号：
- 纸质出版：2017
- 稿件说明：
移动端阅览
王金成, 井明博, 段春燕, 等. 石油烃污染对陇东黄土高原土壤生物学及非生物学特性的影响[J]. 水土保持通报, 2017,37(1):9-16.

WANG Jincheng, JING Mingbo, DUAN Chunyan, et al. Impact of Crude Oil Pollution on Soil Biological and Abiological Properties in Eastern Gansu Province[J]. Bulletin of Soiland Water Conservation, 2017, 37(1): 9-16.
王金成, 井明博, 段春燕, 等. 石油烃污染对陇东黄土高原土壤生物学及非生物学特性的影响[J]. 水土保持通报, 2017,37(1):9-16. DOI： 10.13961/j.cnki.stbctb.2017.01.002.

WANG Jincheng, JING Mingbo, DUAN Chunyan, et al. Impact of Crude Oil Pollution on Soil Biological and Abiological Properties in Eastern Gansu Province[J]. Bulletin of Soiland Water Conservation, 2017, 37(1): 9-16. DOI： 10.13961/j.cnki.stbctb.2017.01.002.

摘要

[目的

]

探索石油污染与土壤生物学及非生物学特性间相互关系，为陇东黄土高原地区石油污染土壤的综合治理提供基础数据和背景资料。[方法

]

采用常规方法测定甘肃省庆阳市5县，1区，8个采油区油污土壤化性质、酶活性和土壤微生物遗传多样性。[结果

]

土壤总石油烃含量（TPHs）与碱解氮、速效磷、pH值和脲酶呈负相关（

0.01），与土壤多酚氧化酶、脱氢酶和过氧化氢酶呈正相关（

0.01）；样地土壤TPHs含量低的土壤微生物Shannon-Wiener指数明显高于TPHs含量高的样地；NMDS排序结果显示，石油污染对该地区土壤影响最大的因子为土壤碱解氮和速效磷，其次为TPHs，pH值、脲酶、Shannon-Wiener指数及过氧化氢酶。[结论

]

陇东地区石油烃污染对土壤环境因子的影响主要表现在包括氮、磷等营养元素的有效循环、土壤脲酶活性和微生物群落多样性，而对土壤微生物群落丰富度、均匀度和速效钾的影响未达到显著水平。

Abstract

[Objective] The objective of this study is to explain relationship between soil biology and abiological characteristics on crude-oil contaminated soil and provide basic data and background information for its comprehensive remediation in eastern Gansu Province. [Methods] The changes of soil physical and chemical properties as well as enzyme activities in crude oil-contaminated soil in Qingyang City of Gansu Province were investigated using the conventional methods

and the genetic diversity of microbial community was analyzed based on PCR-DGGE. [Results] The contents of soil available N

available P

pH value and activity of soil urease decreased with the increase of soil TPHs concentration respectively(p<0.01)

while the activities of soil polyphenoloxidase

dehydrogenase and catalase had a significant positive correlation with soil TPHs concentration respectively(p<0.01). As for the genetic diversity of soil microbial communities

Shannon-Wiener index in the studied sites with lower TPHs concentration was significantly higher than that of the sites with higher TPHs concentration(p<0.05). Moreover

nonmetric multidimensional scaling(NMDS) showed that petroleum-contaminated soil in Gansu Province were strongly affected by soil available N and available P

and following-affected by soil TPHs concentration

pH value

urease activity

Shannon-Wiener index of soil microbial communities and activity of soil catalase.[Conclusion] Impact of crude oil pollution on soil environmental factors in Eastern Gansu Province of Loess Plateau was mainly reflected by the nutrients effective circulation of nitrogen and phosphorus as well as soil microbial-communities diversity and soil urease activity

but there were no significant impact on available K

richness and evenness of soil microbial communities.

关键词

Keywords

references

王金成,周天林,井明博,等.陇东黄土高原地区石油污泥原位修复过程中土壤主要肥力指标动态变化分析[J].环境科学学报,2015,35(1):280-287.

王传远,杨翠云,孙志高,等.黄河三角洲生态区土壤石油污染及其与理化性质的关系[J].水土保持学报,2010,24(2):214-217.

王金成,井明博,段春艳,等.陇东黄土高原石油污染土壤环境因子对金盏菊(

Calendula officinalis

)—微生物联合修复的响应[J].环境科学学报,2015,35(9):2971-2981.

严昶升.土壤肥力研究方法[M].北京:农业出版社,1988:263-280.

唐景春,王斐,褚洪蕊,等.玉米草(

Zea Mexicana

)与海藻寡糖联合修复石油烃污染土壤的研究[J].农业环境科学学报,2010,29(11):2107-2113.

鲍士旦.土壤农化分析[M].3版.北京:中国农业出版社,1999:245-353.

王如刚,王敏,牛晓伟,等.超声—索氏萃取—重量法测定土壤中总石油烃含量[J].分析化学,2010,38(3):417-420.

Shi Guoxi, Liu Yongjun, Johnson N C, et al., Interactive influence of light intensity and soil fertility on root-associated arbuscular mycorrhizal fungi. [J]. Plant and Soil, 2014,378(1/2):173-188.

刘五星,骆永明,滕应,等.石油污染土壤的生态风险评价和生物修复(Ⅱ)：石油污染土壤的理化性质和微生物生态变化研究[J].土壤学报,2007,44(5):848-853.

王金成,井明博,肖朝霞,等.陇东黄土高原地区石油污染土壤微生物群落及其与环境因子的关系[J].水土保持通报,2012,32(5):145-151.

Antonio D A, Francesca B, Massimo G,

et al.,

Bioremediation of petroleum hydrocarbons in anoxicmarine sediments:Consequences on the speciation of heavy metals [J]. Marine Pollution Bulletin, 2009,58(12):1808-1814.

张晓阳,李凯荣,张麟君.陕北石油污染对土壤理化性质的影响[J].水土保持研究,2013,20(3):32-38.

李慧,陈冠雄,杨涛,等.沈抚灌区含油污水灌溉对稻田土壤微生物种群及土壤酶活性的影响[J].应用生态学报, 2005,16(7):1355-1359.

朱凡,洪湘琦,闫文德,等. PAHs污染土壤植物修复对酶活性的影响[J].生态学报,2014,34(3):581-588.

姜睿玲,杨统一,唐玉斌,等.多环芳烃污染对桑园土壤微生物结构及种群多样性的影响[J].中国环境科学,2012,32(9):1655-1661.

Gnseppe T, Maria L C. Guido G. Oxidative polymerisation of phenols by a phenol oxidase from green olives [J]. Enzyme and Microbial technology, 2003,33(1):47-54.

Li Hui, Zhang Chenggang, Cheng Guanxiong, et al., Effect of petroleum-containing wastewater irrigation on bacterial diversities and enzymatic activities in a paddy soil irrigation area [J]. Journal of Environmental Quality, 2005,34(3):1073-1080.

杨茜,吴蔓莉,曹碧霄,等.石油降解菌的筛选、降解特性及其与基因的相关性研究[J].安全与环境学报,2014,14(1):187-192.

Gnseppe T, Maria L C. Guido G. Oxidative polymerisation of phenols by a phenol oxidase from green olives[J]. Enzyme and Microbial technology, 2003,33(1):47-54.

浏览量

1203

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

青铜峡灌区不同改良措施下盐碱化土壤理化性质及微生物群落的变化特征

陇东黄土高原油污土壤环境因子对场地生态修复的季节响应

杉木人工林土壤微团聚体中铁铝氧化物与微生物的分布及其关系

石灰固化黄土土水特征及软化本构模型

基于kNDVI与结构方程模型的洞庭湖区植被覆盖时空变化及驱动机制分析