西北农林科技大学资源环境学院, 农业部西北植物营养与农业环境重点实验室, 陕西杨凌,712100
纸质出版:2014
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伍欢, 王进鑫, 张青, 等. 水分和铅双重胁迫对紫穗槐苗木叶片抗氧化酶活性的影响[J]. 水土保持通报, 2014,33(1):65-69.
WU Huan, WANG Jin-xin, ZHANG Qing, et al. Effects of Combined Drought and Lead Stress on Antioxidant Enzyme Activities of Amorpha Fruticosa L. Seedlings[J]. Bulletin of Soiland Water Conservation, 2014, 33(1): 65-69.
伍欢, 王进鑫, 张青, 等. 水分和铅双重胁迫对紫穗槐苗木叶片抗氧化酶活性的影响[J]. 水土保持通报, 2014,33(1):65-69. DOI: 10.13961/j.cnki.stbctb.2014.01.055.
WU Huan, WANG Jin-xin, ZHANG Qing, et al. Effects of Combined Drought and Lead Stress on Antioxidant Enzyme Activities of Amorpha Fruticosa L. Seedlings[J]. Bulletin of Soiland Water Conservation, 2014, 33(1): 65-69. DOI: 10.13961/j.cnki.stbctb.2014.01.055.
为了探讨在水分和铅双重胁迫条件下
紫穗槐(Amorpha fruticosa L.)苗木叶片抗氧化酶活性的变化
采用旱棚盆栽试验
研究了不同水分胁迫处理(土壤相对含水量分别为田间持水量的100%
80%
60%和40%)和铅胁迫处理(土壤中铅含量分别为0
1000
2000
4000和6000 mg/kg)及其双重作用对紫穗槐苗木叶片的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性的影响。结果表明
在单一的铅胁迫处理下
随着胁迫的加剧
紫穗槐苗木叶片的SOD和CAT活性呈先升高后降低的趋势
POD活性一直呈升高趋势;在单一的水分胁迫处理下
随着胁迫的加剧
SOD和POD活性呈先升高后降低的趋势
CAT活性呈先降低后升高的趋势;水分和铅双重胁迫对SOD和POD活性有显著的激活作用
对低铅含量(≤ 2000 mg/kg)处理的CAT活性有激活作用;适度的双重胁迫(铅含量≤2000 mg/kg
土壤相对含水量≥40%)有利于提高紫穗槐苗木对水分和铅双重胁迫的耐受性。
The objective of this experiment is to study changes of antioxidant enzyme activity in Amorpha fruticosa L. Seedlings grown under combined drought and lead(Pb) stress. Pot experiment was carried out in a rain-proof shed to investigate the effects of drought stress(soil relative water contents were 100%
80%
60% and 40%)
Pb stress(Pb contents were 0
1000
2000
4000 and 6000 mg/kg)
and the combined stress on the superoxide dismutase(SOD)
peroxidase(POD) and catalase(CAT) activities in the leaves of A. fruticosa L. seedlings. Results showed that SOD and CAT activities in the leaves of A. fruticosa L. seedlings first increased and then declined
but POD activity increased with the increase of the Pb content in soil under Pb stress. With the decrease of soil relative water content under drought stress
SOD and POD activities first increased and then declined
and CAT activity first declined and then increased. SOD and POD activities significantly increased under the combined drought and Pb stress
and CAT activity increased under the combined stress when the Pb content in soil was equal to or less than 2000 mg/kg. In conclusion
A. fruticosa L. seedlings have a strong tolerance to the combined effects of drought (soil relative water content≥40%) and Pb (Pb content≤2000 mg/kg) stress.
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