1. 陇东学院生命科学与技术学院甘肃省高校陇东生物资源保护与利用省级重点实验室,甘肃,庆阳,745000
2. 陕西师范大学生命科学学院,陕西,西安,710062
3. 中国科学院水土保持研究所黄土高原土壤侵蚀与旱地农业国家重点实验室, 陕西 杨凌,712100
纸质出版:2012
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王金成, 井明博, 肖朝霞, 等. 陇东黄土高原地区石油污染土壤微生物群落及其与环境因子的关系[J]. 水土保持通报, 2012,31(5):145-151.
WANG Jin-cheng, JING Ming-bo, XIAO Zhao-xia, et al. Relationship Between Microbial-composition of Petroleum-contaminated Soil and Its Environmental Factors in Longdong Region of Loess Plateau[J]. Bulletin of Soiland Water Conservation, 2012, 31(5): 145-151.
王金成, 井明博, 肖朝霞, 等. 陇东黄土高原地区石油污染土壤微生物群落及其与环境因子的关系[J]. 水土保持通报, 2012,31(5):145-151. DOI:
WANG Jin-cheng, JING Ming-bo, XIAO Zhao-xia, et al. Relationship Between Microbial-composition of Petroleum-contaminated Soil and Its Environmental Factors in Longdong Region of Loess Plateau[J]. Bulletin of Soiland Water Conservation, 2012, 31(5): 145-151. DOI:
为了认识和评价石油开采对陇东地区土壤环境的影响
采用平板培养和多元分析法研究了不同石油污染程度下的土壤微生物群落特征及其与土壤环境因子的关系。 结果显示:(1)微生物3大类群的数量以距污染源30m的样地S
1-3
最高
200m的S
7-9
次之
100m的S4-6最低;放线菌数量变化对Shannon-Wiener指数影响较大。 (2)土壤有机质、含油量、含盐量、速效磷均为S
1-3
最高
S
4-6
次之
S
7-9
最低; 土壤pH值、碱解氮及速效钾含量均以S
7-9
最高
S
4-6
次之
S
1-3
最低。 (3)PCA结果显示TPHs含量在8种环境因子中作用最大
其含量的上升导致了土壤含盐率、有机质和速效磷的增加
进而影响到了土壤含水量、pH值、土壤细菌、放线菌、微生物总数及其他环境因子。 研究表明
陇东黄土高原地区开展生物修复石油污染时
应充分开发利用土壤细菌及放线菌资源
并外源投加N
K等营养元素
从而刺激土著石油降解菌的生长
进而提高石油烃的降解效率。
To further understand and assess the effect of oil exploitation on soil environment in the Longdong region of the Loess Plateau
China
soil microbial-community characteristic and its relation with soil environ-mental factors under different oil pollution degrees were investigated by using plate culture and multivariate analysis. Results show that:(1)as for the total number of soil microorganism
the site plots(S1-3)surround-ing oil pollution sources(30m)demonstrated the top
and followed by the site plots(S7-9)
200 maway from the pollution sources
as well as the site plots(S4-6)
100 maway from the pollution sources
demonstrated the bottom. Besides
the group of actinomycetes with a smaller number played a more important role in the change of Shannon-Wiener index. (2)The environmental factors including soil organic matter
TPHs
saltrate and available P in the site plots(S1-3)demonstrated the top but the bottom in the site plots(S7-9); and however
soilp H
available N and available K in the site plots(S7-9)demonstrated the top but the bottom inthe site plots(S1-3). (3)Principal component analysis(PCA)indicated that soil TPHs exerted the greatest in-fluence among the 8 environmental factors
and its increase directly developed the content of soil salt
organic matter and available P
which further affected other environmental factors including soil moisture
pH vaule
bacteria
fungi
actinomycetes and even total microbial amount. The results above revealed that rational de-velopment of soil bacteria and actinomycetes resources and exogenous supply of nutrition concerning N and Kcould stimulate the growth of native oil degradation bacterium and then raise the petroleum hydrocarbon deg-radation efficiency during the process of bio-remediation on petroleum-contaminated soil in the Longdong re-gion of the Loess Plateau
China.
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