Optimization of microbial-enhanced ecological substrate formulations for slope vegetation restoration

Wang Hao; Li Liufang; Yan Huaxiang; Dou Hongqiang; Wu Zhigang

doi:10.13961/j.cnki.stbctb.2025.02.017

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Optimization of microbial-enhanced ecological substrate formulations for slope vegetation restoration

- Optimization of microbial-enhanced ecological substrate formulations for slope vegetation restoration
- Bulletin of Soiland Water Conservation Vol. 45, Issue 2, Pages: 159-170(2025)
- 作者机构：
  
  1. 福州大学紫金地质与矿业学院,福建,福州,350108
  2. 福建省高速公路养护工程有限公司,福建,福州,350005
- 作者简介：
- 基金信息：
- DOI：10.13961/j.cnki.stbctb.2025.02.017
  CLC： X43
- Online First：16 May 2025，
  
  Published：2025
- 稿件说明：
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Wang Hao, Li Liufang, Yan Huaxiang, et al. Optimization of microbial-enhanced ecological substrate formulations for slope vegetation restoration[J]. Bulletin of Soiland Water Conservation, 2025, 45(2): 159-170.
DOI：

Wang Hao, Li Liufang, Yan Huaxiang, et al. Optimization of microbial-enhanced ecological substrate formulations for slope vegetation restoration[J]. Bulletin of Soiland Water Conservation, 2025, 45(2): 159-170. DOI： 10.13961/j.cnki.stbctb.2025.02.017.

摘要

[目的] 探究胶结液浓度、植物纤维、复合微生物肥料及保水剂对微生物固化生态基材强度的提升效果，研究不同配比基材的植物适生性能，筛选出最佳的边坡绿化基材配方，为新型生态基材制备与边坡植被修复提供理论支持和实践指导。[方法] 以胶结液浓度、植物纤维、复合微生物肥料、保水剂为影响因素开展正交试验制备微生物固化生态基材，对比不同配比基材的抗剪强度，并通过高清显微镜分析微生物固化生态基材强度提升的微观机理；采用盆栽试验研究高羊茅和紫花苜蓿在不同配比基材的出苗特征和植被生长情况；基于抗剪强度特征和植物生长情况，通过熵值法筛选最优的基材配方。[结果] ①微生物固化对基材的抗剪强度提升具有显著作用，黏聚力提升179.78%～355.65%，内摩擦角增加35.69%～58.97%。②胶结液浓度是影响植物出芽时间和出苗率最为显著的因素，随着胶结液浓度的升高，出芽时间出现延迟，出苗率明显降低。③基材组植物的生长指标均明显优于对照组，高羊茅在胶结液浓度0.2 mol/L，植物纤维、复合微生物肥料、保水剂质量比为1∶2∶0.09的配比基材中生长发育最好；紫花苜蓿在胶结液浓度0.2 mol/L，植物纤维、复合微生物肥料、保水剂质量比为3∶1∶0.06的配比基材中生长发育最好。[结论] 微生物固化生态基材的抗剪强度较基质土有显著提升，其中0.2 mol/L为最佳胶结液浓度。在该浓度下，适用于高羊茅和紫花苜蓿的最优配比(植物纤维：复合微生物肥料：保水剂)分别为1∶2∶0.09和3∶1∶0.06。

Abstract

[Objective] The effects of cementation solution concentration

plant fibers

microbial fertilizers and superabsorbent polymers on the mechanical and ecological properties of microbial-enhanced ecological substrates were analyzed. The adaptability of plants to substrates with different ratios was investigated，and the best formula of slope greening substrate was selected，in order to provide theoretical support and practical guidance for the preparation of novel ecological substrates and slope vegetation restoration. [Methods] An orthogonal test was performed to evaluate the influence of cement concentration

plant fiber

compound microbial fertilizer and superabsorbent polymers on microbial-enhanced ecological substrate. Thereafter

the shear strength of the substrate with different proportions was compared

and the micro-mechanism of the strength improvement of the microbial-enhanced ecological substrate was analyzed by a high-definition microscope. A pot experiment was conducted to study the seedling emergence characteristics and vegetation growth of tall fescue and alfalfa in different substrate ratios. Based on the characteristics of shear strength and plant growth

the optimal substrate formula was screened using the entropy method. [Results] ① The microbial curing significantly improved the shear strength of the substrate

and increased cohesion and the internal friction angle by 179.78%—355.65% and 35.69%—58.97%

respectively. ② The concentration of cementing solution was the most significant factor affecting the emergence time and rate of plants; as the concentration of the cementing solution increased

the time to emergence increased and the emergence rate decreased significantly. ③ The growth indices of the plants in the substrate group improved significantly compared to those in the control group. The growth and development of tall fescue were best in the substrate with a cementing solution concentration of 0.2 mol/L

and the mass ratio of plant fiber

compound microbial fertilizer

and superabsorbent polymers of 1∶2∶0.09. Alfalfa had the best growth and development in the substrate with a cementing solution concentration of 0.2 mol/L

and plant fiber

compound microbial fertilizer

and superabsorbent polymers mass ratio of 3∶1∶0.06. [Conclusion] The shear strength of microbial-solidified ecological substrates was significantly higher than that of the matrix soil

and 0.2 mol/L was the best cementing solution concentration. At this concentration

the optimal ratios (plant fiber: composite microbial fertilizers: superabsorbent polymers) for tall fescue and alfalfa were 1∶2∶0.09 and 3∶1∶0.06

respectively.

关键词

Keywords

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