Community and functional gene characteristics of soil carbon sequestration microorganisms in Xianshanhu National Wetland Park

Lu Zhangxin; Yan Feiyang; Wu Choufei; Shi Jiayi; Li Peng; Zhao Mingxing; Zhang Xiancui; Zeng Huihui; Zhang Feng

doi:10.13961/j.cnki.stbctb.2025.01.025

您当前的位置：

首页 >

文章列表页 >

Community and functional gene characteristics of soil carbon sequestration microorganisms in Xianshanhu National Wetland Park

- Community and functional gene characteristics of soil carbon sequestration microorganisms in Xianshanhu National Wetland Park
- Bulletin of Soiland Water Conservation Vol. 45, Issue 1, Pages: 235-243(2025)
- 作者机构：
  
  1. 湖州师范学院,浙江,湖州,313000
  2. 浙江省森林资源监测中心,浙江,杭州,310020
  3. 杭州鸿森林业调查规划设计有限公司,浙江,杭州,310020
- 作者简介：
- 基金信息：
- DOI：10.13961/j.cnki.stbctb.2025.01.025
  CLC： Q176;Q178;X171.1
- Published：2025
- 稿件说明：
移动端阅览
Lu Zhangxin, Yan Feiyang, Wu Choufei, et al. Community and functional gene characteristics of soil carbon sequestration microorganisms in Xianshanhu National Wetland Park[J]. Bulletin of Soiland Water Conservation, 2025, 45(1): 235-243.
DOI：

Lu Zhangxin, Yan Feiyang, Wu Choufei, et al. Community and functional gene characteristics of soil carbon sequestration microorganisms in Xianshanhu National Wetland Park[J]. Bulletin of Soiland Water Conservation, 2025, 45(1): 235-243. DOI： 10.13961/j.cnki.stbctb.2025.01.025.

摘要

[目的

]

分析湿地不同土地利用方式下0—20 cm土壤性质和微生物菌群结构变化，探究不同土地利用方式下土壤固碳微生物群落与功能基因变化特征，为湿地生态系统资源的保护和可持续利用提供科学参考。[方法

]

以浙江长兴仙山湖国家湿地公园为研究对象。运用高通量测序技术对基因进行测序分析。[结果

]

①仙山湖湿地固碳能力表现为：莎草沼泽＞天然沿岸森林＞竹林＞苗圃＞人为干预的河道沿岸森林(落羽杉树林)＞人工松树林＞阔叶林＞人为干预的沿岸植被＞人为干预的河道沿岸森林(柳树沼泽)＞芦苇沼泽＞芦苇和柳树沿岸植被，莎草沼泽有机碳储量最高(38.68±0.56 t/hm

)，为森林有机碳储量的171%，其土壤微生物碳利用效率(0.66±0.005)为竹林微生物碳利用效率的150%。②仙山湖湿地微生物固碳途径主要以卡尔文循环，还原性三羧酸循环和还原乙酰辅酶A途径为主，变形菌门、拟杆菌门、酸杆菌门、绿弯菌门为主要固碳菌群。莎草沼泽土壤变形菌门相对丰度为56.40%，拟杆菌门相对丰度为17.40%，天然沿岸森林土壤变形菌门相对丰度为59.60%，竹林土壤酸杆菌门相对丰度为36.00%，仙山湖湿地有机碳储量与土壤固碳菌门丰度成正相关关系。[结论

]

仙山湖湿地生态系统中固碳微生物的优势菌群差异大，不同土地利用方式下土壤固碳微生物群落与功能基因变化影响着湿地微生物固碳能力。其中，莎草沼泽中土壤有机碳储量、全氮含量和微生物碳利用效率最高，其固碳功能基因高于其他土地利用方式。因此，莎草沼泽具有较高的碳储存能力。

Abstract

[Objective] Changes in soil properties and microbial community structure in 0—20 cm soil under different land use modes in wetlands were analyzed

and the characteristics of soil carbon sequestration microbial communities and functional gene changes under different land use modes were explored to provide scientific references for the protection and sustainable utilization of wetland ecosystem resources. [Methods] The Xianshan Lake National Wetland Park in Changxing

Zhejiang Province was taken as the research object. High-throughput sequencing technology was used to sequence the genes. [Results] ① The carbon sequestration capacity of the Xianshan Lake wetland was as follows: sedge swamp>natural coastal forest>bamboo forest>nursery>artificially intervened riverbank forest (Chinese fir forest)>artificial pine forest>broad-leaved forest>artificially intervened coastal vegetation>artificially intervened riverbank forest (willow swamp)>reed swamp>reed and willow coastal vegetation. The organic carbon storage of sedge swamp was the highest (38.68±0.56 t/hm2)

accounting for 171% of forest organic carbon storage

and its soil microbial carbon utilization efficiency (0.66±0.005) was 150% of bamboo forest microbial carbon utilization efficiency. ② The carbon sequestration pathways of microorganisms in the Xianshan Lake wetland were mainly facilitated through the Calvin cycle

reducing the tricarboxylic acid cycle and reducing acetyl CoA pathway

with Proteobacteria

Bacteroidetes

Acidobacteria

and Chloroflexia as the main carbon sequestration microbial communities. The relative abundance of Proteobacteria and Bacteroidetes in the sedge swamp soil were 56.40% and 17.40%

respectively. The relative abundance of Proteobacteria in the natural coastal forest soil was 59.60%

and that of Acidobacteria in the bamboo forest soil was 36.00%. There was a positive correlation between organic carbon storage and soil carbon-fixing bacterial abundance in the Xianshan Lake wetland. [Conclusion] The dominant microbial communities for carbon sequestration in the Xianshan Lake wetland ecosystem varied greatly

and changes in soil carbon sequestration microbial communities and functional genes under different land use patterns affected the carbon sequestration capacity of wetland microorganisms. Among them

soil organic carbon storage

total nitrogen content

and microbial carbon utilization efficiency were the highest in the sedge swamp

and its carbon sequestration functional genes were higher than those of other land-use methods. Therefore

sedge marshes have a high carbon storage capacity.

关键词

Keywords

references

孙欣.灌木扩张对三江平原沼泽湿地植物多样性及其固碳能力的影响[D].黑龙江哈尔滨：东北林业大学,2024. Sun Xin. Effects of shrub expansion on plant diversity and carbon sequestration capacity of marsh wetlands in the Sanjiang Plain [D]. Harbin, Heilongjiang: Northeast Forestry University, 2024.

Saintilan N, Rogers K, Kelleway J J, et al. Climate change impacts on the coastal wetlands of Australia [J]. Wetlands, 2019,39(6):1145-1154.

Gentile R, Vanlauwe B, Six J. Litter quality impacts short-but not long-term soil carbon dynamics in soil aggregate fractions [J]. Ecological Applications, 2011,21(3):695-703.

Mlewski E C, Saona L A, Boidi F J, et al. Exploring soil bacterial diversity in relation to edaphic physicochemical properties of high-altitude wetlands from Argentine Puna [J]. Microbial Ecology, 2023,87(1):6.

段晓男,王效科,逯非,等.中国湿地生态系统固碳现状和潜力[J].生态学报,2008,28(2):463-469. Duan Xiaonan, Wang Xiaoke, Lu Fei, et al. Carbon sequestration and its potential by wetland ecosystems in China [J]. Acta Ecologica Sinica, 2008,28(2):463-469.

Liang Chao, Schimel J P, Jastrow J D. The importance of anabolism in microbial control over soil carbon storage [J]. Nature Microbiology, 2017,2:17105.

刘洋荧,王尚,厉舒祯,等.基于功能基因的微生物碳循环分子生态学研究进展[J].微生物学通报,2017,44(7):1676-1689. Liu Yangying, Wang Shang, Li Shuzhen, et al. Advances in molecular ecology on microbial functional genes of carbon cycle [J]. Microbiology China, 2017,44(7):1676-1689.

Peng Wentao, Wang Yan, Zhu Xiuxiu, et al. Distribution characteristics and diversities of

cbb

and

coxL

genes in paddy soil profiles from Southern China [J]. Pedosphere, 2021,31(6):954-963.

蒋敏,朱培,孙勇,等.仙山湖国家湿地公园湿地类型分析及生态系统研究[J].华东森林经理,2017,31(3):42-45. Jiang Min, Zhu Pei, Sun Yong, et al. Wetland type analysis and ecosystem research of Xianshanhu National Wetland Park [J]. East China Forest Management, 2017,31(3):42-45.

Spohn M, Klaus K, Wanek W, et al. Microbial carbon use efficiency and biomass turnover times depending on soil depth: Implications for carbon cycling [J]. Soil Biology and Biochemistry, 2016,96:74-81.

曾慧慧,蒋敏,赵明星,等.仙山湖国家湿地公园湿地与旱地土壤细菌群落结构特征比较[J].微生物学通报,2024,51(6):1983-1994. Zeng Huihui, Jiang Min, Zhao Mingxing, et al. Comparison of bacterial community structure between wetland and dryland in Xianshan Lake National Wetland Park [J]. Microbiology China, 2024,51(6):1983-1994.

Xu Chonghua, Xu Xia, Ju Chenghui, et al. Long-term, amplified responses of soil organic carbon to nitrogen addition worldwide [J]. Global Change Biology, 2021,27(6):1170-1180.

Solomon D, Lehmann J, Kinyangi J, et al. Long-term impacts of anthropogenic perturbations on dynamics and speciation of organic carbon in tropical forest and subtropical grassland ecosystems [J]. Global Change Biology, 2007,13(2):511-530.

Tan Sijia, Jiang Lijuan, Liu Jingyi, et al. Rhizosphere microorganisms and soil physicochemical properties of restored wetland plant communities at cutting slash of populus deltoides in Dongting Lake [J]. Wetlands, 2023,43(5):48.

Mao Qinggong, Lu Xiankai, Mo Hui, et al. Effects of simulated N deposition on foliar nutrient status, N metabolism and photosynthetic capacity of three dominant understory plant species in a mature tropical forest [J]. Science of the Total Environment, 2018,610:555-562.

韩广轩,李隽永,屈文笛.氮输入对滨海盐沼湿地碳循环关键过程的影响及机制[J].植物生态学报,2021,45(4):321-333. Han Guangxuan, Li Juanyong, Qu Wendi. Effects of nitrogen input on carbon cycle and carbon budget in a coastal salt marsh [J]. Chinese Journal of Plant Ecology, 2021,45(4):321-333.

肖烨,黄志刚,武海涛,等.三江平原典型湿地类型土壤微生物特征与土壤养分的研究[J].环境科学,2015,36(5):1842-1848. Xiao Ye, Huang Zhigang, Wu Haitao, et al. Soil microorganism characteristics and soil nutrients of different wetlands in Sanjinag Plain, Northeast China [J]. Environmental Science, 2015,36(5):1842-1848.

丁俊男,于少鹏,史传奇,等.寒区湿地不同土地利用方式对土壤理化性质和团聚体稳定性的影响[J].生态学杂志,2021,40(11):3543-3551. Ding Junnan, Yu Shaopeng, Shi Chuanqi, et al. Effects of land use types on soil physicochemical properties and aggregates stability in cold region wetland [J]. Chinese Journal of Ecology, 2021,40(11):3543-3551.

Yuan Haoshao, Ge Tida, Wu Xiaohong, et al. Long-term field fertilization alters the diversity of autotrophic bacteria based on the ribulose-1,5-biphosphate carboxy-lase/oxygenase(RubisCO)large-subunit genes in paddy soil [J]. Applied Microbiology and Biotechnology, 2012,95:1061-1071.

Spain A M, Krumholz L R, Elshahed M S. Abundance, composition, diversity and novelty of soil Proteobacteria [J]. The ISME Journal, 2009,3(8):992-1000.

章妮,陈克龙,王欣烨,等.青海湖沼泽湿地cbbM固碳微生物群落对模拟增温的动态响应[J].草地学报,2024,32(12):3752-3763. Zhang Ni, Chen Kelong, Wang Xinye, et al. Dynamic response of the CBBM carbon-fixing microbial community to simulated warming in Qinghai Lake marsh wetland [J]. Acta Prataculturae Sinica, 2024,32(12):3752-3763.

Ma Shengchao, Fang Jiaohui, Liu Jian, et al. Differences in sediment carbon-fixation rate and associated bacterial communities in four wetland types in Hulun Lake Basin [J]. Catena, 2022,213:106167.

张勇,丛军,陆海,等.土壤酸杆菌群落结构与海拔多样性格局[J].环境科学杂志,2014,26(8):1717-1724. Zhang Y, Cong J, Lu H, et al. Community structure and elevational diversity patterns of soil Acidobacteria [J]. Journal of Environmental Sciences, 2014,26(8):1717-1724.

Gao X, Dong S, Xu Y, et al. Revegetation significantly increased the bacterial-fungal interactions in different successional stages of alpine grasslands on the Qinghai-Tibetan Plateau [J]. Catena, 2021,205:105385.

Álvarez J A, Bécares E. The effect of plant harvesting on the performance of a free water surface constructed wetland [J]. Environmental Engineering Science, 2008,25(8):1115-1122.

Yang Chao, Lv Dantong, Jiang Shenyi, et al. Soil salinity regulation of soil microbial carbon metabolic function in the Yellow River Delta, China [J]. Science of the Total Environment, 2021,790:148258.

李子旭,李建华,张强,等.不同复垦措施下土壤微生物群落的响应及组装过程[J].环境科学,2024,45(12):7326-7336. Li Zixu, Li Jianhua, Zhang Qiang, et al. Response and assembly process of soil microbial communities under different reclamation measures [J]. Environmental Science, 2024,45(12):7326-7336.

Campbell B J, Stein J L, Cary S C. Evidence of chemolithoautotrophy in the bacterial community associated with

Alvinella pompejana

, a hydrothermal vent polychaete [J]. Applied and Environmental Microbiology, 2003,69(9):5070-5078.

Paula F S, Rodrigues J L M, Zhou Jizhong, et al. Land use change alters functional gene diversity, composition and abundance in Amazon forest soil microbial communities [J]. Molecular Ecology, 2014,23(12):2988-2999.

赵姣,马静,朱燕峰,等.植被类型对黄土高原露采矿山复垦土壤碳循环功能基因的影响[J].环境科学,2023,44(6):3386-3395. Zhao Jiao, Ma Jing, Zhu Yanfeng, et al. Effects of vegetation types on carbon cycle functional genes in reclaimed soil from open pit mines in the Loess Plateau [J]. Environmental Science, 2023,44(6):3386-3395.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of Drainage Ditches on Biogeochemical Cycling in Wetland－Taking Wetlands in the Sanjiang Plain as an Example

Responses of Plant Community and Soil Fnzyme Activities to Wetland Degradation in the Yellow River Delta Region

Wetland Degradation Characteristics and Restoration Strategies in Fenhe River Watershed of Shanxi Province

Effects of Elevated CO_2 on Soil Organic Carbon and Nitrogen of Wetlands in Sanjiang Plain

Influence of different amendments on salt ions and maize growth in coastal saline soil

Related Author

LU Xian-guo

XI Min

SHAN Qi-hua

FAN Bao-min

XING Shang-jun

ZHANG Jian-feng

ZHANG Feng

PANG Chun-hua

Related Institution

Graduate School of the Chinese Academy of Sciences

Northeast Institute of Geography and Agricultural Ecology, Chinese Academy of Sciences

Institute of Forestry Policy and Information, Chinese Academy of Forestry

Shandong Academy of Forestry

Institute of Subtropical Forestry, Chinese Academy of Forestry

⁰