Effects of Simulated Nitrogen Deposition on Soil Enzyme Activities in Subalpine Pinus Armandii Forest in Central Yunnan Province

Li Xiaodong; Su Lianbo; Wang Keqin; Song Yali; Song Chenggong; Hu Chun

doi:10.13961/j.cnki.stbctb.20240729.002

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Effects of Simulated Nitrogen Deposition on Soil Enzyme Activities in Subalpine Pinus Armandii Forest in Central Yunnan Province

- Effects of Simulated Nitrogen Deposition on Soil Enzyme Activities in Subalpine Pinus Armandii Forest in Central Yunnan Province
- Bulletin of Soiland Water Conservation Vol. 44, Issue 5, Pages: 38-47(2024)
- 作者机构：
  
  1. 西南林业大学生态与环境学院,云南,昆明,650224
  2. 云南省林业和草原科学院漾濞核桃研究院,云南,大理,671000
  3. 西南林业大学水土保持学院,云南,昆明,650224
  4. 国家林业和草原局云南玉溪森林生态系统国家定位观测研究站,云南,昆明,650224
  5. 云南经贸外事职业学院,云南,昆明,650224
- 作者简介：
- 基金信息：
- DOI：10.13961/j.cnki.stbctb.20240729.002
  CLC： S718.55
- Published：2024
- 稿件说明：
移动端阅览
Li Xiaodong, Su Lianbo, Wang Keqin, et al. Effects of Simulated Nitrogen Deposition on Soil Enzyme Activities in Subalpine Pinus Armandii Forest in Central Yunnan Province[J]. Bulletin of Soiland Water Conservation, 2024, 44(5): 38-47.
DOI：

Li Xiaodong, Su Lianbo, Wang Keqin, et al. Effects of Simulated Nitrogen Deposition on Soil Enzyme Activities in Subalpine Pinus Armandii Forest in Central Yunnan Province[J]. Bulletin of Soiland Water Conservation, 2024, 44(5): 38-47. DOI： 10.13961/j.cnki.stbctb.20240729.002.

摘要

[目的

]

探讨亚热带华山松(Pinus armandii)林土壤酶活性的动态规律及其与化学性质关系，为亚热带地区氮沉降研究提供参考。[方法

]

本研究以滇中亚热带华山松林为研究对象，设置对照〔CK，0 g/(m

·a)〕，低氮〔LN， 10 g/(m

·a)〕，中氮〔MN， 20 g/(m

·a)〕和高氮〔HN， 25 g/(m

·a)〕4个氮梯度处理，分析土壤酶活性对氮沉降的响应及其与土壤化学性质之间的关联性。[结果

]

①氮沉降促进了蔗糖酶(16.94%~34.00%)和酸性磷酸酶(23.42%~40.09%)的活性，抑制了脲酶(8.70%~27.18%)和过氧化氢酶 (9.26%~23.36%)的活性，且呈现出显著的时间变化(p＜0.05)；蔗糖酶和脲酶活性分别在氮沉降后第25，27个月(2021年8月，10月)达到最高，酸性磷酸酶和过氧化氢酶活性则在氮沉降后第33个月(2022年6月)达到最高。②土壤pH值、有机碳和全磷含量随氮沉降水平增加而降低，土壤全氮含量则相反，土壤硝态氮和氨态氮含量在MN提高，而在HN降低。③土壤化学性质和酶活性均表现为0—5 cm＞5—10 cm＞10—20 cm的垂直土层分布特征；氮沉降下不同土壤酶活性之间协同效应较高(p＜0.05)；在影响土壤酶活性的环境因子中，土壤全氮是影响蔗糖酶活性的主要敏感因子，土壤有机碳、全磷和氨态氮是影响脲酶和过氧化氢酶活性的主要敏感因子。[结论

]

氮沉降持续增加的情况下，加强了土壤酶之间的协同效应，改变了森林土壤的环境因子，促进或抑制土壤酶活性，进而改变了土壤养分循环。

Abstract

[Objective] The dynamic pattern of soil enzyme activities and its relationship with soil chemical properties in a subtropical Pinus armandii forest was investigated to provide reference for the study of nitrogen deposition in subtropical regions. [Methods] Four nitrogen gradient treatments for the control (CK) and low (LN)

medium (MN)

and high nitrogen (HN) at 0

20 and 25 g/(m2·a)

respectively

were set up in the subtropical Pinus armandii forest of Central Yunnan to analyse the response of soil enzymes to nitrogen deposition and its correlation with soil chemical properties. [Results] ① Nitrogen deposition promoted the activities of invertase (16.94%—34.00%) and acid phosphatase (23.42%—40.09%) and inhibited the activities of urease (8.70%—27.18%) and catalase (9.26%—23.36%)

with temporal variation (p<0.05). Invertase and urease activities were highest at 25th and 27th month after nitrogen deposition (August and October

2021)

whereas acid phosphatase and catalase activities were highest at 33th month after nitrogen deposition (June

2022). ② Soil pH value

organic carbon

and total phosphorus content decreased with increasing nitrogen deposition; in contrary

soil nitrate and ammonia nitrogen content increased in MN and decreased in HN. ③ Soil chemical properties and enzyme activities were characterized by a vertical soil layer distribution of 0—5 cm >5—10 cm >10—20 cm. The synergism between different soil enzyme activities under nitrogen deposition was high (p<0.05)

among the environmental factors affecting soil enzyme activities

soil total nitrogen was the most sensitive factor affecting invertase activity; soil organic carbon

total phosphorus

and ammoniacal nitrogen were the most sensitive factors affecting urease and catalase activities. [Conclusion] With continuously increase in nitrogen deposition

the synergism among soil enzymes was enhanced

the environmental factors affecting forest soil were changed

the soil enzyme activity was promoted or inhibited

and then the soil nutrient cycling was changed too.

关键词

Keywords

references

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