Relationship of Bacterial Microdiversity of Rhizosphere and Bulk Soil with Soil Organic Carbon Mineralization in Maize Fields

Fu Xianheng; Li Shiqing; Yue Shanchao; Shen Yufang

doi:10.13961/j.cnki.stbctb.20230220.001

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Relationship of Bacterial Microdiversity of Rhizosphere and Bulk Soil with Soil Organic Carbon Mineralization in Maize Fields

- Relationship of Bacterial Microdiversity of Rhizosphere and Bulk Soil with Soil Organic Carbon Mineralization in Maize Fields
- Bulletin of Soiland Water Conservation Vol. 43, Issue 1, Pages: 323-331(2023)
- 作者机构：
  
  1. 中国科学院水利部水土保持研究所, 陕西杨凌,712100
  2. 中国科学院大学,北京,100049
  3. 西北农林科技大学资源与环境学院, 陕西杨凌,712100
- 作者简介：
- 基金信息：
- DOI：10.13961/j.cnki.stbctb.20230220.001
  CLC： S513;S154.3
- Published：2023
- 稿件说明：
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Fu Xianheng, Li Shiqing, Yue Shanchao, et al. Relationship of Bacterial Microdiversity of Rhizosphere and Bulk Soil with Soil Organic Carbon Mineralization in Maize Fields[J]. Bulletin of Soiland Water Conservation, 2023, 43(1): 323-331.
DOI：

Fu Xianheng, Li Shiqing, Yue Shanchao, et al. Relationship of Bacterial Microdiversity of Rhizosphere and Bulk Soil with Soil Organic Carbon Mineralization in Maize Fields[J]. Bulletin of Soiland Water Conservation, 2023, 43(1): 323-331. DOI： 10.13961/j.cnki.stbctb.20230220.001.

摘要

[目的] 运用高级别分类学分辨率揭示玉米根际和非根际土壤中细菌群落微多样性，并探讨微多样性与土壤有机碳矿化的关系，从更精细的分类学分辨率水平上为玉米根际土壤中微生物驱动的碳循环提供理论依据。[方法] 以西北农林科技大学曹新庄试验农场为依托，采取田间生长条件下玉米根际和非根际两种土壤类型。利用高通量测序技术，比较OTUs和ASVs两种分类学分辨率水平上玉米根际和非根际土壤中的细菌群落结构，揭示细菌群落的微多样性。同时通过培养试验检测根际和非根际土壤的有机碳矿化特性。[结果] 通过比较OTUs和ASVs两种分类学分辨率水平上的细菌群落，OTUs和ASVs两种方式显示出相似的细菌群落结构。在玉米根际和非根际土壤类型中，ASVs在更高分类学分辨率水平上描绘细菌群落组成，同时揭示了普遍存在于OTUs内的不同菌株或生态型。此外，两种不同生长策略(r-策略和K-策略)细菌物种的相对丰度差异是导致根际和非根际土壤细菌群落结构不同的主要因素。培养试验表明，根际土壤有机碳矿化量显著高于非根际土壤。3 a的连续采样分析结果表明，根系是田间成熟玉米根际和非根际土壤理化性质差异的主要因素而受时间(2019—2021年)的影响较小。根际和非根际土壤细菌微多样性与相应的土壤有机碳累积矿化量具有显著相关性。[结论] ASVs方式展示了田间生长条件下玉米根际和非根际土壤中的细菌微多样性具有显著差异，且细菌微多样性与根际和非根际土壤有机碳累积矿化量相关。

Abstract

[Objective] The bacterial community microdiversity in the maize rhizosphere and bulk soil was determined to study the relationship between microdiversity and soil organic carbon mineralization through the use of high-resolution taxonomy in order to provide a theoretical foundation for microbially driven carbon cycling in the maize rhizosphere. [Methods] The rhizosphere and bulk soil samples were collected in maize fields on the experimental farm of Cao Xinzhuang

Northwest A&F University. High-throughput sequencing technology was used to detect the bacterial community structure in the rhizosphere and bulk soil. Thereafter

distinct taxonomic resolution levels (OTUs vs ASVs) were used to reveal the microdiversity of bacterial communities. Incubation experiments were conducted to examine organic carbon mineralization characteristics of the rhizosphere and bulk soil of maize. [Results] The distinct taxonomic resolution levels revealed similar bacterial community structure in the rhizosphere and bulk soil. ASVs depicted bacterial community composition at a fine scale taxonomic resolution level

and revealed different strains or ecotypes prevalent within the same OTU. Furthermore

differences in the relative abundance of bacterial species from distinct growth strategies (r-and K-strategies) were the main factors contributing to the different bacterial community structures in the rhizosphere and bulk soil. Incubation experiments showed that organic carbon mineralization was significantly higher in the rhizosphere than in bulk soil. The results of three years of continuous sampling revealed that roots were the main factor causing differences between the rhizosphere and bulk soil physicochemical properties of mature maize in the field

and that time had little influence (from 2019 to 2021). Cumulative mineralization of soil organic carbon was associated with bacterial microdiversity between the rhizosphere and bulk soil. [Conclusion] ASV-level exhibited significant differences in bacterial microdiversity between the rhizosphere and bulk soil in a maize field

and bacterial microdiversity was associated with cumulative mineralization of soil organic carbon.

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references

Kuzyakov Y, Razavi B S.Rhizosphere size and shape: Temporal dynamics and spatial stationarity [J]. Soil Biology and Biochemistry, 2019,135:343-360.

张利,邱松,刘建霞,等.植物根际土壤生态研究进展[J].四川农业科技,2021(7):39-40.

姜涛.氮肥运筹对夏玉米产量、品质及植株养分含量的影响[J].植物营养与肥料学报,2013,19(3):559-565.

程扬,刘子丹,沈启斌,等.秸秆生物炭施用对玉米根际和非根际土壤微生物群落结构的影响[J].生态环境学报,2018,27(10):1870-1877.

Fan Kunkun, Cardona C, Li Yuntao, et al. Rhizosphere-associated bacterial network structure and spatial distribution differ significantly from bulk soil in wheat crop fields [J]. Soil Biology and Biochemistry, 2017,113:275-284.

Peiffer J A, Spor A, Koren O, et al. Diversity and heritability of the maize rhizosphere microbiome under field conditions [J]. Proceedings of the National Academy of Sciences of the United States of America, 2013,110(16):6548-6553.

高贵锋,褚海燕.微生物组学的技术和方法及其应用[J].植物生态学报,2020,44(4):395-408.

钟辉,刘亚军,王滨花,等.分析方法对细菌群落16S rRNA基因扩增测序分析结果的影响[J].生物技术通报,2022,38(6):81-92.

Koeppel A F, Wu M. Surprisingly extensive mixed phylogenetic and ecological signals among bacterial Operational Taxonomic Units [J]. Nucleic Acids Research, 2013,41(10):5175-5188.

Larkin A A, Martiny A C. Microdiversity shapes the traits, niche space, and biogeography of microbial taxa [J]. Environmental Microbiology Reports, 2017,9(2):55-70.

Callahan B J, McMurdie P J, Holmes S P. Exact sequence variants should replace operational taxonomic units in marker-gene data analysis [J]. The ISME Journal, 2017,11(12):2639-2643.

Martiny A C, Treseder K, Pusch G.Phylogenetic conservatism of functional traits in microorganisms [J]. The ISME Journal, 2013,7(4):830-838.

Smith P, Fang Changming, Dawson J J C, et al. Impact of global warming on soil organic carbon [M]. Advances in Agronomy. Amsterdam: Elsevier, 2008:1-43.

Blagodatskaya E, Kuzyakov Y. Mechanisms of real and apparent priming effects and their dependence on soil microbial biomass and community structure: Critical review [J]. Biology and Fertility of Soils, 2008,45(2):115-131.

莫朝阳,张鑫林,杨京平.根际激发效应对土壤有机碳累积及分解的影响[J].浙江大学学报(农业与生命科学版),2021,47(4):527-533.

李晓月.根际土壤不同形态碳、氮的含量及特性研究[D].陕西杨凌:西北农林科技大学,2011.

杨泽良,李萍芳,薛涛,等.玉米根际土壤微生物群落结构及多样性季节变化特征[J].西南农业学报,2020,33(5):1001-1010.

Page A L, Miller R H, Keeney D R. Methods of soil analysis (part Ⅱ)[J]. American Society of Agronomy, Madison, WI, 1982.

Cui Yongxing, Bing Haijian, Fang Linchuan, et al. Diversity patterns of the rhizosphere and bulk soil microbial communities along an altitudinal gradient in an alpine ecosystem of the Eastern Tibetan Plateau [J]. Geoderma, 2019,338:118-127.

Lundin D, Severin I, Logue J B, et al. Which sequencing depth is sufficient to describe patterns in bacterial α-and β-diversity? [J]. Environmental Microbiology Reports, 2012,4(3):367-372.

García-García N, Tamames J, Linz A M, et al. Microdiversity ensures the maintenance of functional microbial communities under changing environmental conditions [J]. The ISME Journal, 2019,13(12):2969-2983.

Glassman S I, Martiny J B H. Broadscale ecological patterns are robust to use of exact sequence variants versus operational taxonomic units [J]. mSphere, 2018,3(4):e00148-e00118.

Huang Yuhong, Liu Yue, Geng Jun, et al. Maize root-associated niches determine the response variation in bacterial community assembly and function to phthalate pollution [J]. Journal of Hazardous Materials, 2022,429:128280.

Fierer N, Bradford M A, Jackson R B. Toward an ecological classification of soil bacteria [J]. Ecology, 2007,88(6):1354-1364.

Philippot L, Raaijmakers J M, Lemanceau P, et al. Going back to the roots: The microbial ecology of the rhizosphere [J]. Nature Reviews Microbiology, 2013,11(11):789-799.

Di Cello F, Bevivino A, Chiarini L, et al. Biodiversity of a Burkholderia cepacia population isolated from the maize rhizosphere at different plant growth stages [J]. Applied and Environmental Microbiology, 1997,63(11):4485-4493.

Dalmastri C, Chiarini L, Cantale C, et al. Soil type and maize cultivar affect the genetic diversity of maize root-associated Burkholderia cepacia populations [J]. Microbial Ecology, 1999,38(3):273-284.

Foster K R, Schluter J, Coyte K Z, et al. The evolution of the host microbiome as an ecosystem on a leash [J]. Nature, 2017,548(7665):43-51.

Fu Xianheng, Song Qilong, Li Shiqing, et al. Dynamic changes in bacterial community structure are associated with distinct priming effect patterns [J]. Soil Biology and Biochemistry, 2022,169:108671.

Kandeler E, Marschner P, Tscherko D, et al. Microbial community composition and functional diversity in the rhizosphere of maize [J]. Plant and Soil, 2002,238(2):301-312.

黄耀,刘世梁,沈其荣,宗良纲.环境因子对农业土壤有机碳分解的影响[J].应用生态学报,2002,13(6):709-714.

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