Microbial Mechanism of Helianthus Tuberosus Root Exudates for Improving Saline-alkali Soil

Yang Min; Li Shuai; Cao Huixiang; Huang Ting; Li Yuan; Xie Junwei; Zhao Gengmao

doi:10.13961/j.cnki.stbctb.2021.06.011

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Microbial Mechanism of Helianthus Tuberosus Root Exudates for Improving Saline-alkali Soil

- Microbial Mechanism of Helianthus Tuberosus Root Exudates for Improving Saline-alkali Soil
- Bulletin of Soiland Water Conservation Vol. 41, Issue 6, Pages: 71-81(2021)
- 作者机构：
  
  南京农业大学资源与环境科学学院/江苏省海洋生物学重点实验室,江苏,南京,210095
- 作者简介：
- 基金信息：
- DOI：10.13961/j.cnki.stbctb.2021.06.011
  CLC： S156.4
- Published：2021
- 稿件说明：
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Yang Min, Li Shuai, Cao Huixiang, et al. Microbial Mechanism of Helianthus Tuberosus Root Exudates for Improving Saline-alkali Soil[J]. Bulletin of Soiland Water Conservation, 2021, 41(6): 71-81.
DOI：

Yang Min, Li Shuai, Cao Huixiang, et al. Microbial Mechanism of Helianthus Tuberosus Root Exudates for Improving Saline-alkali Soil[J]. Bulletin of Soiland Water Conservation, 2021, 41(6): 71-81. DOI： 10.13961/j.cnki.stbctb.2021.06.011.

摘要

[目的

]

探究菊芋在滨海盐土改良过程中的作用机制，分析菊芋和碱蓬根系分泌物的组分差异，明确土壤微生态环境的变化规律，进一步为盐土改良提供理论依据。[方法

]

以种植菊芋和自然碱蓬植被为样地，对菊芋和碱蓬的根系分泌物进行对比分析，研究在根系分泌物作用下土壤微生物数量，微生物量碳氮，微生物群落结构以及土壤酶活性的变化，从而系统地阐明根系分泌物介导下盐土改良的微生物机制。[结果

]

菊芋根际土壤中含有果糖（2.343×10

-3

g/kg）、葡萄糖（4.235×10

-3

g/kg）、蔗糖（2.670×10

-3

g/kg），分别是碱蓬根际土壤的9.28，1.52和2.43倍。而菊芋根际与非根际中的果糖含量存在显著性差异（p

0.05），其根际中含量为非根际的12.02倍。菊芋土壤还含有低聚果糖（蔗果三糖、蔗果四糖和蔗果五糖），而碱蓬土壤中未检测出低聚果糖。除糖类外，菊芋根系分泌物还含有烷烃、酚、醛、酯、有机酸、醇、酮、酰胺，其组分较碱蓬土壤更为复杂且某些组分为菊芋特有〔1-氯—十八烷、正十六烷酸、2-甲基-Z-4-十四碳烯、十二酮、（Z）-9-十八碳酰胺、苯丙酸十六烷基酯等〕。功能性根系分泌物（如低聚果糖、果糖、十六烷、十八烷酸等）为根际微生物提供碳源、氮源和营养元素的同时，使菊芋根际土壤中微生物数量显著增加（p

0.05），土壤微生物量碳、氮显著高于碱蓬土壤（p

0.05），其值分别是碱蓬土壤的1.95和1.6倍，且菊芋根际的微生物量碳、氮约为非根际的1.69和1.50倍，优势菌群（变形菌门、放线菌门、绿弯菌门、酸杆菌门）所占比重达到90%，土壤有益菌群（Actinobacteria和Acidobacteria）的相对丰度显著增加（p

0.05），土壤生物活性提升。此外，菊芋根际特有的分泌物（十六烷、烯醛等），抑制了病原菌的生长，优化了微生物群落结构。除过氧化氢酶外，土壤脲酶、蔗糖酶和碱性磷酸活性显著提高（p

0.05），其活性分别是碱蓬土壤的1.83，1.88和3.30倍。[结论

]

种植菊芋后，通过根际分泌物介导，改善土壤微生物群落结构与功能，增加土壤酶活性，使土壤生物活力得以整体提升，与原生植被碱蓬相比，降低了土壤含盐量，起到了改良盐土的作用。

Abstract

[Objective] The mechanism of Helianthus tuberosus in the improvement of coastal saline soil was explored

the compositional differences of root exudates of Helianthus tuberosus and Suaeda salsa was analyzed

and the changing laws of the soil micro-ecological environment were clarified

in order to provide a theoretical basis for saline soil improvement.[Methods] The root exudates of Helianthus tuberosus and Suaeda salsa were compared and analyzed

and the changes of soil microbial quantity

microbial biomass carbon and nitrogen

microbial community structure and soil enzyme activity under the action of root exudates were studied

so as to systematically clarify the microbial mechanism of saline soil improvement mediated by root exudates.[Results] The rhizosphere soil of Helianthus tuberosus contained fructose (2.343×10-3 g/kg)

glucose (4.235×10-3 g/kg)

and sucrose (2.67×10-3 g/kg)

which were 9.28

1.52

2.43 times of that in Suaeda salsa rhizosphere soil. The difference between the content of fructose in the rhizosphere and non-rhizosphere of Helianthus tuberosus was significant (p<0.05)

and the content in the rhizosphere was 12.02 times of that in the non-rhizosphere. Helianthus tuberosus soil also contained oligofructose (sucrose triose

sucrose tetraose and sucrose pentaose)

but no fructooligosaccharides were detected in the soil of Suaeda salsa. In addition to sugars

Helianthus tuberosus root exudates also contained alkanes

phenols

aldehydes

esters

organic acids

alcohols

ketones

and amides. Its components were more complex than Suaeda salsa soil and some components were unique to Helianthus tuberosus[1-Chloro-octadecane

n-hexadecanoic acid

2-methyl-Z-4-tetradecene

dodecone

(Z)-9-octadecyl amide

and hexadecyl phenylpropionate Esters.] . Functional root exudates (such as oligofructose

fructose

hexadecane

and octadecanoic acid.) provided carbon source

nitrogen source and nutrient elements for rhizosphere microorganisms. The number of microorganisms in the rhizosphere soil of Helianthus tuberosus increased significantly (p<0.05)

and the soil microbial biomass carbon and nitrogen were significantly higher than that in Suaeda salsa soil (p<0.05)

which were 1.95 and 1.6 times of that in Suaeda salsa soil

and the microbial biomass carbon and nitrogen of Jerusalem artichoke rhizosphere were about 1.69 times and 1.50 times higher than that in non-rhizosphere soil. And the dominant flora (Proteobacteria

Actinomycota

Chloroflexum

Acidobacteria) accounted for 90%. The relative abundance of soil beneficial bacteria (Actinobacteria and Acidobacteria) increased significantly

and soil biological activity was significantly improved. In addition

the unique secretions of Helianthus tuberosus rhizosphere (hexadecane

and enal.) inhibited the growth of pathogenic bacteria and optimized the microbial community structure. Except for catalase

the activities of soil urease

invertase and alkaline phosphate were significantly increased (p<0.05)

and their activities were 1.83 times

1.88 times and 3.30 times higher than those in Suaeda salsa soil

respectively.[Conclusion] After planting Jerusalem artichoke

through the mediation of rhizosphere exudates

the structure and function of soil microbial community were improved

soil enzyme activity was increased

and soil biological activity was improved as a whole. Compared with the native vegetation Suaeda salsa

it reduces the salt content of the soil and plays a role in improving saline soil.

关键词

Keywords

references

Mirjam H B, Kroes J G, Ruud P B, et al. Quantification of the impact of hydrology onagricultural production as a result of too dry, too wet or too saline conditions[J]. Soil, 2016, 2(3):391-402.

Panagea I S, Daliakopoulos I N, Tsanis I K, et al. Evaluation of promising technologies for soil salinity amelioration in Timpaki(Crete):A participatory approach[J]. Solid Earth, 2016, 7(1):177-190.

Yang Hui, Hu Jinxiang, Long Xiaohua, et al. Salinity altered root distribution and increased diversity of bacterial communities in the rhizosphere soil of Jerusalem artichoke[J]. Scientific Reports, 2016, 6(1):20687.

Wang W, Vinocur B, Altman A. Plant responses to drought, salinity and extreme temperatures:towards genetic engineering for stress tolerance[J]. Planta, 2003, 218(1):1-14.

王佳丽, 黄贤金, 钟太洋, 等.盐碱地可持续利用研究综述[J].地理学报, 2011, 66(5):673-684.

Li Jiangguo, Pu Lijie, Han Mingfang, et al. Soil salinization research in China:Advances and prospects[J]. Journal of Geographical Sciences, 2014, 24(5):943-960.

Li Lianqing, Wang Dan, Liu Xiaoyu, et al. Soil organic carbon fractions and microbial community and functions under changes in vegetation:A case of vegetation succession in karst forest[J]. Environmental Earth Sciences, 2014, 71(8), 3727-3735.

Alvarez B, Dioselina C. Improvement of saline soils with

Vicia sativa

L. from a semiarid region[J]. Bragantia, 2018, 77(3):501-509.

Banaras N, Jelte R. Improvement of saline soil productivity through farm yard manure, amendment and coated seeds for fodderbeet cultivation[J]. Asian Journal of Plant Sciences, 2002, 1(2):82-84.

Li Zhigang, Liu Xiaojing, Zhang Xiumei. Infiltration of melting saline ice water in soil columns:consequences on soil moisture and salt content[J]. Agricultural Water Management, 2008, 95(4):498-502.

Hooper D, Lavelle P, van der Putten W, et al. Interactions between above and below ground biodiversity in terrestrial eeosystems:Pattems, mechanisms, and feedbacks[J]. Bioscience, 2000, 50(2):1049-1061.

殷博.几种作物根系分泌物对土壤微生物的影响[D].黑龙江哈尔滨:黑龙江大学, 2009.

郭立泉.星星草抗碱生理适应机制的研究[D].吉林长春:东北师范大学, 2009.

朱丽霞, 章家恩, 等.根系分泌物与根际微生物相互作用研究综述[J].生态环境, 2001, 2(1):102-105.

谷益安.土壤细菌群落和根系分泌物影响番茄青枯病发生的生物学机制[D].江苏南京:南京农业大学, 2017.

刘丹梅, 姜吉禹, 杨君.菊芋的生态功能研究[J].北方园艺, 2009(10):140-142.

胡涛, 刘泽鑫, 张晓, 等.改良东北滨海盐碱地的优良作物-菊芋[J].农村经济与科技, 2017, 28(11):62-63.

Long Xiaohua, Zhao Jie, Liu Zhaopu, et al. Applying geostatistics to determine the soil quality improvement by Jerusalem artichoke in coastal saline zone[J]. Ecological Engineering, 2014, 70(1):319-326.

Long Xiaohua, Shao Hongbo, Liu Ling, et al. Jerusalem artichoke:A sustainable biomass feedstock for biorefinery[J]. Renewable and Sustainable Energy Reviews, 2016, 54(1):1382-1388.

Joy E J M, Broadley M R, Young S D, et al. Soil type influences crop mineral composition in Malawi[J]. Science of the Total Environment, 2015, 505(1):587-595.

Chenery S R, Izquierdo M, Marzouk E, et al. Soil plant interactions and the uptake of Pb at abandoned mining sites in the Rookhope catchment of the N. Pennines, UK:A Pb isotope study[J]. Science of the Total Environment, 2012, 433(1):547-560.

Zheng Jie, Li Gairu, Cheng Yufeng, et al. Determination of the content of oligofructose in thorn sugar by HPLC/ESI-MS method[J]. Chinese New Drugs and Clinical Pharmacology, 2015, 26(5):671-675.

Wang Hao, Liu Yanqin, Yang Hongmei, et al. Determination of fructose, glucose, sucrose, maltose and lactose in sugar-free food by liquid chromatography-mass spectrometry[J]. Chinese Journal of Analytical Chemistry, 2010, 38(6):873-876.

王艳颖, 胡文忠, 庞坤, 马垄.高效液相色谱-蒸发光散射法测定苹果中可溶性糖的含量[J].食品与发酵工业, 2008, 45(6):129-131.

Mo Jixian, Wang Zhigang, Wang Changhe. Comparison of common microbial quantity determination methods[J]. Biology Teaching, 2012, 37(1):42-43.

Brookes P C, Landman A, Pruden G, et al. Chloroform fumigation and the release of soil nitrogen:A rapid direct extraction method to measure microbial biomass nitrogen in soil[J]. Soil Biology & Biochemistry, 1985, 17(6):837-842.

王学翠, 童晓茹, 温学森, 等.植物与根际微生物关系的研究进展[J].山东科学, 2007(6):40-50.

胥九兵, 王乃强, 刘宗利, 等.菊芋资源开发利用研究进展[J].精细与专用化学品, 2012, 20(11):18-20.

Yang Wanqin, Zhong Zhangcheng, Tao Jianping, et al. The relationship between forest soil enzyme activity and plant diversity in Jinyun Mountain[J]. Forestry Science, 2001, 37(4):124-128.

杨慧.盐胁迫对菊芋根系及根际土壤主要特征影响的研究[D].江苏南京:南京农业大学, 2016.

Rao A V. Dose-response effects of inulin and oligofructose on intestinal bifidogenesis effects[J]. The Journal of Nutrition, 1999, 129(7):1442-1445.

Shen Jianbao, Zhang Fusao, Huang Qin, et al. Determination of organic acids in plant root exudates by high performance liquid chromatography[J]. Pedosphere, 1999, 8(1):45-52.

周牮君, 王校常, 吴文彬.根系分泌物对几种难溶磷活化作用的研究[J].西南农业大学学报, 2001, 23(5):401-403.

Chikkerur J, Samanta A K, Kolte A P, et al. Production of short chain fructo-oligosaccharides from inulin of chicory root using fungal endoinulinase[J]. Applied Biochemistry and Biotechnology, 2020, 191(2):695-715.

Bouhnik Y, Raskine L, Simoneau G, et al. The capacity of short-chain fructo-oligosaccharides to stimulate faecal bifidobacteria:A dose-response relationship study in healthy humans[J]. Nutrition Journal, 2006, 28(5):8-13.

Yuan Renwen, Liu Lin, Zhang Rui, et al. Research progress on the mechanism of interaction between plant rhizosphere exudates and soil microorganisms[J]. Chinese Agricultural Science Bulletin, 2020, 36(2):26-35.

Sykes E G, Skinner F A. Actinomycetales:Characteristics and practical importance[M]. Academic, 1973, 24(1):339.

Liu Junjie, Sui Yueyu, Yu Zhenhua, et al. Diversity and distribution patterns of acidobacterial communities in the black soil zone of Northeast China[J].2016, 95(21):212-222.

Murray A H, Iason G R, Stewart C. Effect of simple phenolic compounds of heather(

Calluna vulgaris

) on rumen microbial activity in vitro[J]. Journal of Chemical Ecology, 1996, 22(8):1493-1504.

Ola A, Doda I C, Quinton J N. Can we manipulate root system architecture to control soul erosion[J]. Soil, 2015, 1(2):603-612.

杨生伟.保护地草莓连作土壤微生物区系及酶活性变化研究[D].甘肃兰州:甘肃农业大学, 2007.

Poulami C, Sandipan S, Rangasamy A, et al. Beneficial soil bacterium pseudomonas frederiksbergensis OS261 augments salt tolerance and promotes red pepper plant growth[J]. Frontiers in Plant Science, 2017, 8(1):705-713.

张建锋, 张旭东, 周金星, 等.世界盐碱地资源及其改良利用的基本措施[J].水土保持研究, 2005, 12(6):28-30.

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