Effects of Degradation of Alpine Meadow on Soil Microbial Genes in Nitrogen Transformation in Qinghai-Tibet Plateau

Wang Xiangtao; Zhang Chao; Liao Lirong; Wang Jie; Yu Lanhui; Zhang Xianzhou

doi:10.13961/j.cnki.stbctb.2020.03.002

您当前的位置：

首页 >

文章列表页 >

Effects of Degradation of Alpine Meadow on Soil Microbial Genes in Nitrogen Transformation in Qinghai-Tibet Plateau

- Effects of Degradation of Alpine Meadow on Soil Microbial Genes in Nitrogen Transformation in Qinghai-Tibet Plateau
- Bulletin of Soiland Water Conservation Vol. 40, Issue 3, Pages: 8-13(2020)
- 作者机构：
  
  1. 西藏农牧学院动物科学学院,西藏,林芝,860000
  2. 中国科学院地理科学与资源研究所, 生态系统网络观测与模拟重点实验室, 拉萨高原生态试验站,北京,100101
  3. 中国科学院大学,北京,100049
  4. 西北农林科技大学水土保持研究所, 陕西杨凌,712100
  5. 中国科学院大学资源与环境学院,北京,100190
- 作者简介：
- 基金信息：
- DOI：10.13961/j.cnki.stbctb.2020.03.002
  CLC： S812.2
- Published：2020
- 稿件说明：
移动端阅览
Wang Xiangtao, Zhang Chao, Liao Lirong, et al. Effects of Degradation of Alpine Meadow on Soil Microbial Genes in Nitrogen Transformation in Qinghai-Tibet Plateau[J]. Bulletin of Soiland Water Conservation, 2020, 40(3): 8-13.
DOI：

Wang Xiangtao, Zhang Chao, Liao Lirong, et al. Effects of Degradation of Alpine Meadow on Soil Microbial Genes in Nitrogen Transformation in Qinghai-Tibet Plateau[J]. Bulletin of Soiland Water Conservation, 2020, 40(3): 8-13. DOI： 10.13961/j.cnki.stbctb.2020.03.002.

摘要

[目的] 深入分析高寒草甸退化过程中土壤氮素转化特征，明确草甸退化对土壤氮素转化微生物基因丰度的影响，为认识高寒草甸的退化机理以及科学治理高寒退化草甸提供重要依据。[方法] 以青藏高原不同退化程度高寒草甸（未退化、轻度退化、中度退化、重度退化）为研究对象，利用实时定量PCR法分析退化过程中土壤理化性质及与氮素转化相关基因（nifH，amoA-AOA，amoA-AOB，narG，nirK，nirS和nosZ）丰度的变化，明确影响高寒草甸氮素转化基因的关键因子。[结果] ①随退化程度的加剧，高寒草甸土壤有机碳、全氮、硝态氮及铵态氮含量逐渐降低；②高寒草甸退化降低了与氮素转化相关的固氮nifH基因、氨氧化amoA-AOA和amoA-AOB基因丰度，但增加了反硝化narG，nirS和nirK基因丰度，且在重度退化草甸丰度最高；③nifH，amoA-AOA和amoA-AOB基因与土壤有机碳、硝态氮、铵态氮及水分呈显著正相关，narG，nirS和nirK基因与土壤有机碳、硝态氮及铵态氮含量呈显著负相关，与pH值呈显著正相关。[结论] 高寒草甸退化对氮素转化微生物具有重要影响，土壤有机碳、pH值及水分是影响土壤氮素转化微生物基因的主要因素。

Abstract

[Objective] The characteristics of nitrogen (N) transformation in degraded alpine meadows and identify the effects of meadow degradation on the gene abundance of soil nitrogen-transforming microorganisms were studied thoroughly

in order to provide a basis for understanding the degradation mechanism and scientific management of alpine meadows.[Methods] A soil survey along a degraded gradient

including undegraded

lightly degraded

moderately degraded

and severely degraded meadows

in the Qinghai-Tibetan alpine region was conducted. The changes in soil physicochemical properties and genes in N transformation (including nifH

amoA-AOA

amoA-AOB

narG

nirK

nirS

and nosZ) were investigated by using real-time quantitative PCR method

and the drivers of these functional genes were clarified.[Results] ① The contents of soil organic carbon (SOC)

total N

nitrate N

and ammonium N decreased along the degradation gradient. ② Degradation led to a significant reduction in the abundance of nifH

amoA-AOA

and amoA-AOB genes

whereas it caused an increase in the abundance of narG

nirS

and nirK genes

which was the maximum in the severely degraded meadow soils. ③ The abundance of the nifH

amoA-AOA

and amoA-AOB genes was significantly positively correlated with the contents of SOC

nitrate N

ammonium N

and soil moisture. The abundance of the narG

nirS

and nirK genes was significantly negatively correlated with the contents of SOC

nitrate N

and ammonium N

whereas it was positively correlated with the pH value.[Conclusion] The degradation of the alpine meadows had an important influence on the microorganisms involved in N transformation. The SOC

pH value

and moisture were the significant drivers of the functional genes in soil nitrogen-transforming microorganisms in alpine meadow soils.

关键词

Keywords

references

字洪标,胡雷,阿的鲁骥,等.不同退化演替阶段高寒草甸群落根土比和土壤理化特征分布格局[J].草地学报,2015,23(6):1151-1160.

樊博,林丽,曹广民,等.不同退化阶段高寒草甸土壤物理性质与植物根系的相互关系[J].生态学报,2020,40(7):1-10.

马源,李林芝,张德罡,等.高寒草甸根际土壤化学计量特征对草地退化的响应[J].应用生态学报,2019,30(9):3039-3048.

Nannipieri P, Eldor P. The chemical and functional characterization of soil N and its biotic components [J]. Soil Biology & Biochemistry, 2009,41(12):2357-2369.

Zhang Chao, Song Zilin, Zhuang Daohua, et al. Urea fertilization decreases soil bacterial diversity, but improves microbial biomass, respiration, and N-cycling potential in a semiarid grassland [J]. Biology and Fertility of Soils,2019,55(3):229-242.

Nelson M B, Berlemont R, Martiny A C, et al. Nitrogen cycling potential of a grassland litter microbial community [J]. Applied and Environmental Microbiology, 2015,81(20):7012-7022.

郝爱华,薛娴,彭飞,等.青藏高原典型草地植被退化与土壤退化研究[J].生态学报,2020,40(3):464-975.

蔡晓布,张永青,邵伟.不同退化程度高寒草原土壤肥力变化特征[J].生态学报,2008,28(3):1034-1044.

胡雷,王长庭,王根绪,等.三江源区不同退化演替阶段高寒草甸土壤酶活性和微生物群落结构的变化[J].草业学报,2014,23(3):8-19.

蒋永梅,师尚礼,田永亮,等.高寒草地不同退化程度下土壤微生物及土壤酶活性变化特征[J].水土保持学报,2017,31(3):244-249.

Xue Xian, You Quangang, Peng Fei, et al. Experimental warming aggravates degradation-induced topsoil drought in alpine meadows of the Qinghai-Tibetan Plateau [J]. Land Degradation & Development, 2017,28(8):2343-2353.

鲁如坤.土壤与农业化学分析[M].北京:中国农业出版社, 1999.

廖李容,王杰,张超,等. 禁牧对半干旱草地土壤氮循环功能基因丰度和氮储量的影响[J]. 应用生态学报,2019,30(10):3473-3481.

Zhang Chao, Xue Sha, Liu Guobin, et al. A comparison of soil qualities of different revegetation types in the Loess Plateau,China [J]. Plant and Soil, 2011,347(1):163-178.

王玉琴,尹亚丽,李世雄.不同退化程度高寒草甸土壤理化性质及酶活性分析[J].生态环境学报,2019,28(6):1108-1116.

郭小伟,杜岩功,林丽,等.青藏高原北缘3种高寒草地的CH

、CO

和N

O通量特征的初步研究[J].草业科学,2016,33(1):27-37.

肖翔,格日才旦,侯扶江.青藏高原放牧和地形对高寒草甸群落

多样性和土壤物理性质的影响[J].草业科学,2019,36(12):3041-3051.

林璐,乌云娜,田村宪司,等.呼伦贝尔典型退化草原土壤理化与微生物性状[J].应用生态学报,2013,24(12):3407-3414.

高海宁,张勇,秦嘉海,等.祁连山黑河上游不同退化草地有机碳和酶活性分布特征[J].草地学报,2014,22(2):283-290.

陈银萍,罗永清,李玉强,等. 兰州地区农田土壤速效磷与速效钾含量的变化特征[J]. 水土保持通报,2014,34(4):46-52.

Jung J, Yeom J, Kim J, et al. Change in gene abundance in the nitrogen biogeochemical cycle with temperature and nitrogen addition in Antarctic soils [J]. Research in Microbiology, 2011,162(10):1018-1026.

Pan Hong, Ying Shanshan, Liu Haiyang, et al. Microbial pathways for nitrous oxide emissions from sheep urine and dung in a typical steppe grassland [J]. Biology and Fertility of Soils, 2018,54(6):717-730.

Barta J, Melichova T, Vanek D, et al. Effect of pH and dissolved organic matter on the abundance of nir

and nir

denitrifiers in spruce forest soil [J]. Biogeochemistry, 2010,101(1),123-132.

Xu Yuqing, Wan Shiqiang, Cheng Weixin, et al. Impacts of grazing intensity on denitrification and N

O production in a semi-arid grassland ecosystem [J]. Biogeochemistry, 2008,88(2):103-115.

Mergel A, Kloos K, Bothe H. Seasonal fluctuations in the population of denitrifying and N

-fixing bacteria in an acid soil of a Norway spruce forest [J]. Plant and Soil, 2001,230(1):145-160.

Views

924

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Soil wind erosion law in degraded bare patches in alpine meadows of Qinghai Province

Assessment on Contents of Heavy Metals and Properties of Apple Orchard Soil with Different Planting Ages in Luochuan County

Spatial Heterogeneity of Nitrogen Distribution in Sediment of Dianchi Lake

Effects of Soil and Water Conservation Measures on Soil Properties in the Low Mountain and Hill Area of Jinl in Province

Effects of Nitrogen Input on Characteristics of Nitrogen Loss from Sloping Fields Under Natural Rainfall in Central Yunnan Province

Related Author

Tong Shengchun

Long Yuxiang

Zhu Haili

Liu Yabin

Li Jinfang

Zhao Jianyun

Li Xilai

Hu Xiasong

Related Institution

State Key Laboratory of Three-River Source;Ecology and Plateau Agriculture and Animal Husbandry， Qinghai University

Key Laboratory of Cenozoic Resource & Environment in Northern Margin of the;Tibetan Plateau

Department of Geological Engineering， Qinghai University

Institute of Earth Environment, State Key Laboratory of Loess and Quaternary Geology, Chinese Academy of Sciences, Xian

College of Tourism and Environmental Science, Shaanxi Normal University, Xian

⁰