Isolation of Plant Growth Promoting Bacillus Spp. and Their Impact on Feldspathic Sandstone Soil Characteristics

Deng Lin; Wang Tao; Yin Tutong; He Linyan; Yang Caiqian; Liang Zhishui

doi:10.13961/j.cnki.stbctb.2019.05.029

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Isolation of Plant Growth Promoting Bacillus Spp. and Their Impact on Feldspathic Sandstone Soil Characteristics

- Isolation of Plant Growth Promoting Bacillus Spp. and Their Impact on Feldspathic Sandstone Soil Characteristics
- Bulletin of Soiland Water Conservation Vol. 39, Issue 5, Pages: 211-217(2019)
- 作者机构：
  
  1. 东南大学土木工程学院,江苏,南京,211189
  2. 农业部农业环境微生物重点实验室南京农业大学生命科学学院,江苏,南京,210095
- 作者简介：
- 基金信息：
- DOI：10.13961/j.cnki.stbctb.2019.05.029
  CLC： Q93;S156;S182
- Published：2019
- 稿件说明：
移动端阅览
Deng Lin, Wang Tao, Yin Tutong, et al. Isolation of Plant Growth Promoting Bacillus Spp. and Their Impact on Feldspathic Sandstone Soil Characteristics[J]. Bulletin of Soiland Water Conservation, 2019, 39(5): 211-217.
DOI：

Deng Lin, Wang Tao, Yin Tutong, et al. Isolation of Plant Growth Promoting Bacillus Spp. and Their Impact on Feldspathic Sandstone Soil Characteristics[J]. Bulletin of Soiland Water Conservation, 2019, 39(5): 211-217. DOI： 10.13961/j.cnki.stbctb.2019.05.029.

摘要

[目的] 筛选砒砂岩土壤中植物促生芽孢杆菌，为微生物强化植物改良砒砂岩土壤的科学设想提供资源和技术基础。[方法] 以产生植物激素IAA（indole acetic acid）、铁载体和生物膜为筛选指标，从内蒙古砒砂岩区土壤和植物样品中筛选植物促生芽孢杆菌，采用盆栽试验探究植物促生芽孢杆菌改良砒砂岩土壤特性和促进苜蓿和黑麦草生长的作用。[结果] 筛选到的12株芽孢杆菌产生IAA、铁载体和生物膜的能力不同，分别属于Bacillus halotolerans，B.atrophaeus，B.siamensis和B.zhangzhouensis种群。与对照相比，B.halotolerans P75能够显著增加砒砂岩土壤的有机质含量（24.7%）、速效磷含量（11.9%）和速效钾含量（21.0%）等养分指标，每1 g土壤可培养细菌达到7.4 lg CFU，土壤蔗糖酶活性显著增强（58.8%）。接种B.halotolerans P75后，在砒砂岩土壤中生长的苜蓿和黑麦草干重增加22.3%~81.5%，[结论] 从生长于砒砂岩土壤的苜蓿根内筛选到B.halotolerans P75，可提高砒砂岩土壤肥力，促进苜蓿和黑麦草生长，具有强化植物改良砒砂岩土壤的潜力。

Abstract

[Object] The characteristics of the plant growth promoting Bacillus spp. in a feldspathic sandstone soil were investigated in order to provide resources and technical information for feldspathic sandstone soil improvement.[Methods] Bacillus spp. was isolated from plants and feldspathic sandstone soil in a region of Inner Mongolia. The bacterium were identified by 16S rDNA sequence analysis. The ability of Bacillus spp. for producing indole acetic acid (IAA)

siderophores

and biofilms were tested. Greenhouse experiments were conducted to evaluate the effect of Bacillus spp. on the quality of feldspathic sandstone soil

and on the growth of alfalfa (Medicogo sativa) and perennial ryegrass (Lolium perenne).[Results] Twelve strains of Bacillus spp. were screened and had different production of IAA

siderophores

and biofilms

which belong to B. halotolerans

B. atrophaeus

B. siamensis and B. zhangzhouensis. The isolated B. halotolerans P75 was able to significantly increase the content of soil organic matter (24.7%)

available phosphorus (11.9%)

and available potassium (21.0%) in the sandstone compared with the non-inoculated control. Soil invertase activity (58.8%) and the number of culturable bacteria (7.4 lg CFU in 1 g soil) also significantly increased after B. halotolerans P75 inoculation. Moreover

B. halotolerans P75 significantly increased the dry weight of alfalfa and perennial ryegrass grown on the feldspathic sandstone soil by 22.3%~81.5% in comparison to those of the control.[Conclusion] Our results suggest that the B. halotolerans P75

isolated from the alfalfa roots growing in feldspathic sandstone area

can increase the fertility of feldspathic sandstone soil and promote the growth of alfalfa and ryegrass

and has great potential for the phytoremediation and improvement of feldspathic sandstone soil.

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