Effects of Covering or Burying Trifolium Repens on Diversity of Soil Bacteria Community in an Apple Orchard in Loess Plateau

Ma Zhenzhu; Wei Qianqian; Li Shangwei; Yang Wenquan; Kou Jiancun

doi:10.13961/j.cnki.stbctb.2019.05.020

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Effects of Covering or Burying Trifolium Repens on Diversity of Soil Bacteria Community in an Apple Orchard in Loess Plateau

- Effects of Covering or Burying Trifolium Repens on Diversity of Soil Bacteria Community in an Apple Orchard in Loess Plateau
- Bulletin of Soiland Water Conservation Vol. 39, Issue 5, Pages: 143-151(2019)
- 作者机构：
  
  1. 西北农林科技大学草业与草原学院, 陕西杨凌,712100
  2. 西北农林科技大学生命科学学院, 陕西杨凌,712100
- 作者简介：
- 基金信息：
- DOI：10.13961/j.cnki.stbctb.2019.05.020
  CLC： S182;S154.3
- Published：2019
- 稿件说明：
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Ma Zhenzhu, Wei Qianqian, Li Shangwei, et al. Effects of Covering or Burying Trifolium Repens on Diversity of Soil Bacteria Community in an Apple Orchard in Loess Plateau[J]. Bulletin of Soiland Water Conservation, 2019, 39(5): 143-151.
DOI：

Ma Zhenzhu, Wei Qianqian, Li Shangwei, et al. Effects of Covering or Burying Trifolium Repens on Diversity of Soil Bacteria Community in an Apple Orchard in Loess Plateau[J]. Bulletin of Soiland Water Conservation, 2019, 39(5): 143-151. DOI： 10.13961/j.cnki.stbctb.2019.05.020.

摘要

[目的] 探究白三叶在不同降解时期果园土壤细菌多样性的变化，为果园白三叶的利用及果园生草模式的推广提供理论依据。[方法] 在陕西省洛川县苹果园内分别以覆盖和埋置两种方式将白三叶（Trifolium repens）返园，通过高通量测序研究两种返园方式下白三叶降解1，3，6，12个月时土壤细菌的多样性、丰度及群落结构。[结果] ①白三叶返园处理下，土壤微生物优势菌群种类未表现出显著变化，丰度存在差异，返园处理组土壤中出现梭杆菌门（Acidobacteria）、广古菌门（Euryarchaeota）、泉古菌门（Crenarchaeota）、螺旋体门（Spirochaetes）和硝化螺旋菌（Nitrospira）门等特有菌群。②白三叶返园对苹果园土壤细菌群落分布存在显著影响（p<0.05），且不同降解时期存在差异。两种返园处理组中酸杆菌门（Acidobacteria）、拟杆菌门（Bacteroidetes）、变形菌门（Proteobacteria）丰度增高，厚壁菌门（Firmicutes）丰度降低。③随着白三叶降解时间增加，芽单胞菌（Gemmatimonas）、黄杆菌（Flavobacterium）、Opitutu和Arenimonas属的细菌相对丰度增高，硝化螺旋菌属（Nitrospira）、奈瑟菌属（Neisseria）、Pirellula和Steroidobacter的丰度降低。[结论] 覆盖和埋置白三叶均能改善土壤菌群结构，提高有益菌丰度，促进土壤微生物生态系统物质循环。

Abstract

[Objective] The return of Trifolium repens to an apple orchard was investigated to understand the effects on soil bacteria diversity in different degradation stages

and to provide theoretical basis for the utilization of T. repens in orchards and the promotion of pasture-planting in orchards.[Methods] Fresh T. repens was covered or buried in an apple orchard in Luochuan County

Shannxi Province. At 1

6 and 12 months of T. repens degradation

soil samples were collected

and high-throughput sequencing technology was performed to determine the diversity

richness and structure of soil bacteria.[Results] ① There were no significant differences in the diversity of preponderant bacteria after T. repens returning

but some richness was different. Acidobacteria

Euryarchaeota

Crenarchaeota

Spirochaetes and Nitrospira were found in the soil of the return treatment group. ② There were some remarkable differences in community distribution of soil bacteria (p<0.05)

varying in different degrading period after the return of T. repens. The richness of Acidobacteria

Bacteroidetes and Proteobacteria increased

but that of Firmicutes decreased in soil samples from the two treatment groups compared with the control. ③ Over time

the richness of Gemmatimonas

Flavobacterium

Opitutu

and Arenimonas increased in groups in which T. repens had returned; however

the richness of Nitrospira

Neisseria

Pirellula

and Steroidobacter decreased.[Conclusion] Either covering or burying T. repens could optimize structure of soil bacteria community

increase probiotics abundance and improve the matter cycle in the microbial ecosystem.

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