Response of Seed Germination of Lycium Ruthenicum to PEG-Simulated Drought Stress

GUO Youyan; YU Hongyuan; KONG Dongsheng; ZHANG Yajuan; WU Jiayu

doi:10.13961/j.cnki.stbctb.2017.05.017

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Response of Seed Germination of Lycium Ruthenicum to PEG-Simulated Drought Stress

- Response of Seed Germination of Lycium Ruthenicum to PEG-Simulated Drought Stress
- Bulletin of Soiland Water Conservation Vol. 37, Issue 5, Pages: 98-102(2017)
- 作者机构：
  
  1. 河西学院河西学院黑果枸杞工程中心,甘肃,张掖,734000
  2. 河西学院甘肃省河西走廊特色资源利用重点实验室,甘肃,张掖,734000
  3. 甘肃农业大学林学院,甘肃,兰州,730070
- 作者简介：
- 基金信息：
- DOI：10.13961/j.cnki.stbctb.2017.05.017
  CLC：
- Published：2017
- 稿件说明：
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GUO Youyan, YU Hongyuan, KONG Dongsheng, et al. Response of Seed Germination of Lycium Ruthenicum to PEG-Simulated Drought Stress[J]. Bulletin of Soiland Water Conservation, 2017, 37(5): 98-102.
DOI：

GUO Youyan, YU Hongyuan, KONG Dongsheng, et al. Response of Seed Germination of Lycium Ruthenicum to PEG-Simulated Drought Stress[J]. Bulletin of Soiland Water Conservation, 2017, 37(5): 98-102. DOI： 10.13961/j.cnki.stbctb.2017.05.017.

摘要

[目的] 研究干旱胁迫对黑果枸杞种子萌发及幼苗生长的影响，为黑果枸杞人工栽培提供理论依据。[方法] 通过聚乙二醇（polyethylene glycol，PEG）模拟干旱胁迫，测定不同干旱胁迫强度下黑果枸杞种子的萌发进程、发芽率、发芽势、发芽指数、活力指数、幼苗的生长、幼苗渗透调节物质、叶绿素含量等指标，研究干旱胁迫对黑果枸杞种子萌发及幼苗生长的影响。[结果] 干旱胁迫不利于黑果枸杞种子的萌发，重度干旱胁迫推迟其萌发的高峰期；干旱胁迫下黑果枸杞种子的发芽率、发芽势均呈下降趋势，-0.6 MPa处理下，黑果枸杞种子发芽率、发芽势相对对照分别降低72.06%和74.07%；干旱胁迫对黑果枸杞种子发芽指数和活力指数有抑制作用，其抑制程度随干旱胁迫强度的增加而增加；适度干旱胁迫有利于黑果枸杞幼苗胚根和胚芽的生长，而中度、重度干旱胁迫不利于幼苗胚芽的生长；干旱胁迫下黑果枸杞幼苗根、芽脯氨酸含量和可溶性糖含量均随着胁迫程度的增加逐渐增加，而幼苗叶绿素含量（Chl a+b）随干旱胁迫强度的增加逐渐降低。[结论] 干旱胁迫对黑果枸杞种子萌发及幼苗生长有重要影响，适度的干旱胁迫有利于黑果枸杞幼苗的生长。

Abstract

[Objective] To study the response of seed germination and seedling growth of Lycium ruthenicum to PEG(polyethylene glycol)-simulated drought stress

and to provide a theoretical basis for artificial cultivation of L. ruthenicum.[Methods] PEG-6000 solution was used to simulate drought stress. Seed germination progress

germination percentage

germination potential

germination index

vital index

seedling growth

seedling osmotic adjustment substances

and chlorophyⅡ content under water stress were recorded. The effects of drought stress on seed germination and seedling growth of L. ruthenicum were studied.[Results] Drought stress was disadvantaged to seed germination of L. ruthenicum

and the severe drought stress will delay the fastigium of L. ruthenicum seed germination. Seed germination percentage and germination potential decreased with the increasing level of drought stress. Under -0.6 MPa treatment

germination percentage and germination potential decreased by 72.06% and 74.07% as compared to the one of control treatment. Germination index and vital index were restrained under drought stress

and the restraining degree increased with the increasing of drought stress. The moderate drought stress was beneficial to the growth of radicle and germ

however

it wasn't beneficial to the growth of germ under medium and severe drought stress. Proline and soluble sugar of seedling root and shoot increased with the increasing drought stress level. However

chlorophyⅡ content decreased with the increasing drought stress level.[Conclusion] Drought stress had important effect to the seed germination and seedling growth of L. ruthenicum

and the moderate drought stress was beneficial to the growth of L. ruthenicum seedling.

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