Impact of Crude Oil Pollution on Soil Biological and Abiological Properties in Eastern Gansu Province

WANG Jincheng; JING Mingbo; DUAN Chunyan; WANG Guofeng; SHI Guoxi; ZHOU Tianlin

doi:10.13961/j.cnki.stbctb.2017.01.002

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Impact of Crude Oil Pollution on Soil Biological and Abiological Properties in Eastern Gansu Province

- Impact of Crude Oil Pollution on Soil Biological and Abiological Properties in Eastern Gansu Province
- Bulletin of Soiland Water Conservation Vol. 37, Issue 1, Pages: 9-16(2017)
- 作者机构：
  
  1. 甘肃省高校陇东生物资源保护与利用省级重点实验室,甘肃,庆阳,745000
  2. 陇东学院生命科学与技术学院,甘肃,庆阳,745000
  3. 陕西师范大学生命科学学院,陕西,西安,710062
  4. 中国科学院西北高原生物研究所,青海,西宁,810008
- 作者简介：
- 基金信息：
- DOI：10.13961/j.cnki.stbctb.2017.01.002
  CLC：
- Published：2017
- 稿件说明：
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WANG Jincheng, JING Mingbo, DUAN Chunyan, et al. Impact of Crude Oil Pollution on Soil Biological and Abiological Properties in Eastern Gansu Province[J]. Bulletin of Soiland Water Conservation, 2017, 37(1): 9-16.
DOI：

WANG Jincheng, JING Mingbo, DUAN Chunyan, et al. Impact of Crude Oil Pollution on Soil Biological and Abiological Properties in Eastern Gansu Province[J]. Bulletin of Soiland Water Conservation, 2017, 37(1): 9-16. DOI： 10.13961/j.cnki.stbctb.2017.01.002.

摘要

[目的

]

探索石油污染与土壤生物学及非生物学特性间相互关系，为陇东黄土高原地区石油污染土壤的综合治理提供基础数据和背景资料。[方法

]

采用常规方法测定甘肃省庆阳市5县，1区，8个采油区油污土壤化性质、酶活性和土壤微生物遗传多样性。[结果

]

土壤总石油烃含量（TPHs）与碱解氮、速效磷、pH值和脲酶呈负相关（

0.01），与土壤多酚氧化酶、脱氢酶和过氧化氢酶呈正相关（

0.01）；样地土壤TPHs含量低的土壤微生物Shannon-Wiener指数明显高于TPHs含量高的样地；NMDS排序结果显示，石油污染对该地区土壤影响最大的因子为土壤碱解氮和速效磷，其次为TPHs，pH值、脲酶、Shannon-Wiener指数及过氧化氢酶。[结论

]

陇东地区石油烃污染对土壤环境因子的影响主要表现在包括氮、磷等营养元素的有效循环、土壤脲酶活性和微生物群落多样性，而对土壤微生物群落丰富度、均匀度和速效钾的影响未达到显著水平。

Abstract

[Objective] The objective of this study is to explain relationship between soil biology and abiological characteristics on crude-oil contaminated soil and provide basic data and background information for its comprehensive remediation in eastern Gansu Province. [Methods] The changes of soil physical and chemical properties as well as enzyme activities in crude oil-contaminated soil in Qingyang City of Gansu Province were investigated using the conventional methods

and the genetic diversity of microbial community was analyzed based on PCR-DGGE. [Results] The contents of soil available N

available P

pH value and activity of soil urease decreased with the increase of soil TPHs concentration respectively(p<0.01)

while the activities of soil polyphenoloxidase

dehydrogenase and catalase had a significant positive correlation with soil TPHs concentration respectively(p<0.01). As for the genetic diversity of soil microbial communities

Shannon-Wiener index in the studied sites with lower TPHs concentration was significantly higher than that of the sites with higher TPHs concentration(p<0.05). Moreover

nonmetric multidimensional scaling(NMDS) showed that petroleum-contaminated soil in Gansu Province were strongly affected by soil available N and available P

and following-affected by soil TPHs concentration

pH value

urease activity

Shannon-Wiener index of soil microbial communities and activity of soil catalase.[Conclusion] Impact of crude oil pollution on soil environmental factors in Eastern Gansu Province of Loess Plateau was mainly reflected by the nutrients effective circulation of nitrogen and phosphorus as well as soil microbial-communities diversity and soil urease activity

but there were no significant impact on available K

richness and evenness of soil microbial communities.

关键词

Keywords

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