Effects of Oxytetracycline and Cd Pollution on Soil Respiration and Enzyme Activity

YAN Lei; BI Shi-xin; ZHAO Qi-hui; WANG Zhe; PAN Dong-qi; QU Juan-juan

doi:10.13961/j.cnki.stbctb.2014.06.026

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Effects of Oxytetracycline and Cd Pollution on Soil Respiration and Enzyme Activity

- Effects of Oxytetracycline and Cd Pollution on Soil Respiration and Enzyme Activity
- Bulletin of Soiland Water Conservation Vol. 33, Issue 6, Pages: 101-108(2014)
- 作者机构：
  
  东北农业大学资源与环境学院寒地黑土资源利用与保护重点实验室,黑龙江,哈尔滨,150030
- 作者简介：
- 基金信息：
- DOI：10.13961/j.cnki.stbctb.2014.06.026
  CLC：
- Published：2014
- 稿件说明：
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YAN Lei, BI Shi-xin, ZHAO Qi-hui, et al. Effects of Oxytetracycline and Cd Pollution on Soil Respiration and Enzyme Activity[J]. Bulletin of Soiland Water Conservation, 2014, 33(6): 101-108.
DOI：

YAN Lei, BI Shi-xin, ZHAO Qi-hui, et al. Effects of Oxytetracycline and Cd Pollution on Soil Respiration and Enzyme Activity[J]. Bulletin of Soiland Water Conservation, 2014, 33(6): 101-108. DOI： 10.13961/j.cnki.stbctb.2014.06.026.

摘要

随着饲料工业以及畜禽养殖业的规模化发展

抗生素和重金属在土壤环境中同时存在的几率不断增大。为了分析抗生素和重金属对土壤微生物生态系统的影响

以土霉素(OTC)与镉(Cd)为污染物

采用室内培养法

研究了土霉素(OTC)与镉(Cd)单一处理及复合污染对土壤呼吸和酶活性的影响。结果表明

10 mg/kg重金属镉单独污染对土壤微生物呼吸表现为先抑制后激活作用

且显著抑制了土壤蔗糖酶、脲酶、磷酸酶活性

对3种酶活性平均抑制率从大到小依次为:蔗糖酶 >磷酸酶 >脲酶;1 mg/kg土霉素显著激活土壤微生物呼吸

50和200 mg/kg土霉素对土壤微生物呼吸的影响呈现出先抑制后激活的规律。各处理浓度下的土霉素对蔗糖酶和脲酶活性均主要表现为抑制作用

对磷酸酶活性的影响呈现出一定的波动性;当土霉素的浓度为1和200 mg/kg时

其与10 mg/kg镉的复合污染对土壤微生物呼吸及3种酶活性的影响主要为拮抗作用

但当土霉素的浓度为50 mg/kg时

与10 mg/kg镉的复合污染对土壤微生物呼吸及3种酶活性的影响则主要为协同作用。微生物呼吸对土霉素与镉胁迫更为敏感

最高抑制率和激活率分别可达98.98%和300.82%

土壤酶活性受土霉素和镉污染的影响要弱于它们对土壤微生物呼吸的影响。

Abstract

Co-existence probability of antibiotics and heavy metals in soil environment increased with the large-scale development of feed and livestock industry. In order to analyze the effects of antibiotics and heavy metals on soil microorganism ecosystem

we studied the single or combined pollution of oxytetracycline(OTC) and cadmium(Cd) on soil respiration and enzyme activities by laboratory cultivation. The results showed that the soil respiration was inhibited firstly

and then stimulated by sole treatment of 10 mg/kg Cd

while activities of invertase

urease and phosphatase in soil were significantly inhibited during the whole process. The order of inhibiting rate for enzyme activity was invertase >phosphatase >urease. Soil respiration was significantly activated by sole treatment of OTC at concentration of 1 mg/kg

while inhibited first

then activated by OTC at concentration of 50 or 200 mg/kg. Activities of invertase and urease were inhibited but the activities of phosphatase fluctuated when oxytetracycline was solely used. The combined pollution treatments of OTC+Cd(1+10 mg/kg) as well as OTC+Cd(200+10 mg/kg) showed antagonistic action

while the treatment of OTC+Cd(50+10 mg/kg) presented synergistic action on soil respiration and the 3 kinds of enzyme activities. Soil respiration responded more sensitively than soil enzyme activity by combined pollution of OTC and Cd

with the maximum inhibition and activation rates of 98.98% and 300.82%

respectively. Effects of OTC and Cd on enzyme activities were less than that on soil respiration.

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