Differences in Physiological Responses of Avena Nuda Seedlings to NaCl and KCl Stress

LIU Jian-xin; WANG Jin-cheng; WANG Rui-juan; JIA Hai-yan

doi:10.13961/j.cnki.stbctb.2014.05.020

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Differences in Physiological Responses of Avena Nuda Seedlings to NaCl and KCl Stress

- Differences in Physiological Responses of Avena Nuda Seedlings to NaCl and KCl Stress
- Bulletin of Soiland Water Conservation Vol. 33, Issue 5, Pages: 74-79(2014)
- 作者机构：
  
  陇东学院生命科学与技术学院甘肃省高校陇东生物资源保护与利用省级重点实验室,甘肃,庆阳,745000
- 作者简介：
- 基金信息：
- DOI：10.13961/j.cnki.stbctb.2014.05.020
  CLC：
- Published：2014
- 稿件说明：
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LIU Jian-xin, WANG Jin-cheng, WANG Rui-juan, et al. Differences in Physiological Responses of Avena Nuda Seedlings to NaCl and KCl Stress[J]. Bulletin of Soiland Water Conservation, 2014, 33(5): 74-79.
DOI：

LIU Jian-xin, WANG Jin-cheng, WANG Rui-juan, et al. Differences in Physiological Responses of Avena Nuda Seedlings to NaCl and KCl Stress[J]. Bulletin of Soiland Water Conservation, 2014, 33(5): 74-79. DOI： 10.13961/j.cnki.stbctb.2014.05.020.

摘要

为探讨燕麦对NaCl和KCl胁迫的生理响应差异

采用水培法

研究了不同浓度NaCl和KCl胁迫对幼苗生长

活性氧代谢和渗透调节的影响。结果表明:(1)在75和150 mmol/L浓度下

NaCl胁迫对燕麦幼苗的膜脂过氧化伤害和生长抑制大于KCl胁迫。NaCl胁迫下叶片中的超氧化物岐化酶(SOD)

过氧化氢酶(CAT)活性及可溶性蛋白、可溶性糖和脯氨酸含量低于KCl胁迫;当浓度增大到225 mmol/L时

KCl胁迫叶片中O

丙二醛(MDA)

可溶性蛋白和可溶性糖含量显著大于NaCl胁迫

而SOD

抗坏血酸过氧化物酶(APX)活性及谷胱甘肽(GSH)含量则相反。(2) 225 mmol/L KCl和NaCl处理的植株叶片水势分别为-0.867和-1.034 MPa

渗透势分别为-1.409和-1.252 MPa

说明KCl对燕麦的更强伤害不是渗透胁迫所致;经225 mmol/L KCl胁迫后

燕麦叶片中Na

含量下降至对照的36.5%

而K

含量上升为对照的1.49倍

而补充20 mmol/L NaCl显著提高了225 mmol/L KCl胁迫下叶片Na

的含量及SOD

APX活性

降低了K

.和MDA含量

说明离子毒害引起的活性氧积累可能是高浓度KCl胁迫对燕麦幼苗伤害大于NaCl胁迫的重要原因。

Abstract

In order to explore the differences in physiological responses of oat(Avena nuda L.) seedlings to NaCl and KCl stress

the changes in seedling growth

reactive oxygen species metabolism and osmotic adjustment in leaves of oat seedlings under NaCl and KCl stress of different concentration were investigated by hydroponics method. The results showed that:(1) At the concentration of 75 mmol/L and 150 mmol/L

NaCl stress had greater inhibition effect on the seedling growth and greater damage to the membrane lipid peroxidation than that of KCl stress

the activities of superoxide dismutase(SOD) and catalase(CAT) and the contents of soluble protein

soluble sugar and proline in the seedling leaves of NaCl stress were lower than that under KCl stress; The contents of O2-.

H2O2

malondialdehyde(MDA)

soluble protein and soluble sugar under KCl stress were much higher than that under NaCl stress when the concentration increase to 225 mmol/L

but the activities of SOD and ascorbate peroxidase(APX) and the glutathione(GSH) content of KCl stress were lower than NaCl stress.(2) After treated with 225 mmol/L of KCl and NaCl

the leaf water potential was-0.867 MPa and-1.034 MPa. Besides

the osmotic potential was-1.409 MPa and-1.252 MPa

respectively

indicating that the increased damage caused by 225 mmol/L of KCl was not due to the osmotic stress. Under the stress of 225 mmol/L KCl

the leaf Na+ content decreased by 36.5% and K+ content increased by 1.49 times

compared with the control

while the supplement of 20 mmol/L NaCl could increase the Na+ content and the activities of SOD and APX

decrease the contents of K+

H2O2

O2-.and MDA in leaves of oat seedlings under 225 mmol/L KCl stress significantly. The active oxygen accumulation induced by ion toxicity in oat seedling leaves could be responsible for the enhanced damage caused by 225 mmol/L of KCl than that by NaCl.

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Keywords

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