Effect of Karst Physiognomy on Soil Microbial Abundance and Enzyme Activities

LI Qiang; JIN Zhen-jiang; LI Zhong-yi; LUO Kun; TANG Zhi-qin; HUANG Jing-yun; LU Wen-ti

doi:10.13961/j.cnki.stbctb.2014.03.011

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Effect of Karst Physiognomy on Soil Microbial Abundance and Enzyme Activities

- Effect of Karst Physiognomy on Soil Microbial Abundance and Enzyme Activities
- Bulletin of Soiland Water Conservation Vol. 33, Issue 3, Pages: 50-54(2014)
- 作者机构：
  
  1. 国土资源部/广西岩溶动力学重点实验室中国地质科学院岩溶地质研究所,广西,桂林,541004
  2. 广西环境污染控制理论与技术重点实验室桂林理工大学环境科学与工程学院,广西,桂林,541004
  3. 广西农业科学院农业资源与环境研究所,广西,南宁,530007
- 作者简介：
- 基金信息：
- DOI：10.13961/j.cnki.stbctb.2014.03.011
  CLC：
- Published：2014
- 稿件说明：
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LI Qiang, JIN Zhen-jiang, LI Zhong-yi, et al. Effect of Karst Physiognomy on Soil Microbial Abundance and Enzyme Activities[J]. Bulletin of Soiland Water Conservation, 2014, 33(3): 50-54.
DOI：

LI Qiang, JIN Zhen-jiang, LI Zhong-yi, et al. Effect of Karst Physiognomy on Soil Microbial Abundance and Enzyme Activities[J]. Bulletin of Soiland Water Conservation, 2014, 33(3): 50-54. DOI： 10.13961/j.cnki.stbctb.2014.03.011.

摘要

以中国地质科学院岩溶地质研究所桂林丫吉岩溶试验场为研究区

单氨加氧酶的编码基因amoA的部分序列作为氨氧化细菌(AOB)指示基因

利用荧光定量PCR技术

结合平板菌落计数法和土壤酶活性测定

探讨了岩溶地貌部位及土壤成因对微生物群落丰度和酶活性的影响。结果发现

受岩溶地貌部位和土壤成因的影响

土壤酶活性、土壤微生物总数在垭口、坡地、洼地呈增加的趋势

而氨氧化细菌丰度则呈现降低的趋势。结果表明

土壤氨氧化细菌丰度与真菌数呈极其显著的正相关

而与蔗糖酶活性呈极其显著负相关。

Abstract

The Yaji experimental site of Institute of Karst Geology

Chinese Academy of Geology Science

was selected as the study area to discuss the effects of karst physiognomy on soil microbial abundance and enzyme activity. The gene abundances of amoA

as the index of ammonia-oxidizing bacteria(AOB) communities

were determined by real-time quantitative PCR(qPCR)

while microbial community population and soil enzymes were analyzed using dilute plate incubation counting and colorimetric methods

respectively. Results show that the enzyme activities and total microbial numbers of soils in saddle back

piedmont slope and karst depression increased while the soil AOB decreased. Moreover

the abundances of AOB communities had a significantly positive correlation with fungi

while it had a significantly negative correlation with sucrase activity.

关键词

Keywords

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